| 利用微卫星标记分析鸵鸟遗传多样性 |
| 投稿时间：2009-04-01 修订日期：2009-10-20 点此下载全文 |
| 引用本文：王咏絮 ，任战军，王 乐，王洪亮.利用微卫星标记分析鸵鸟遗传多样性[J].西北农业学报,2010,19(3):36~39 |
| DOI:10.7606/j.issn.1004-1389.2010.03.008 |
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| 基金项目:陕西省农业攻关项目（2005K01 G7 02）。 |
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| 中文摘要:随机采取无直接亲缘关系的68只鸵鸟血样，采用微卫星标记技术对鸵鸟20个微卫星座位进行遗传多样性检测。结果表明，有3个座位（CAU13、CAU36、CAU46）没有多态性，其他17个座位（LEI0094、CAU5、CAU7、CAU8、CAU11、CAU14、CAU15、CAU16、CAU17、CAU22、CAU23、CAU24、CAU32、CAU34、CAU42、CAU43、和CAU57）共检测到142个等位基因，平均每个座位上等位基因数为8.3529±1.2803，平均期望杂合度及多态信息含量分别为0.8468±0.1368和0.8213±0.0321。鸵鸟群体内存在丰富的遗传变异，遗传多样性较高。 |
| 中文关键词:鸵鸟 微卫星标记 遗传多样性 多态信息含量 |
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| Genetic Diversity in the Population of Ostrich Analyzed by Microsatellite Markers |
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| Abstract:In order to study ostrich’s genetic characteristics on the molecular level，68 individuals without direct relationship were picked out according to the random sampling method. The genetic diversity was analyzed using 20 microsatellite markers. The results showed that a total of 142 alleles were detected in 17 microsatellite loci (LEI0094，CAU5，CAU7，CAU8，CAU11，CAU14，CAU15，CAU16，CAU17，CAU22，CAU23，CAU24，CAU32，CAU34，CAU42，CAU43 and CAU57 )，excepting 3 non-polymorphic microsatellite loci (CAU13，CAU36，CAU46)，the number of alleles per locus was 8.3529±1.2803，the average expected heterozygosity and the average value of polymorphism information content（PIC）were 0.8468±0.1368 and 0.8213±0.0321 respectively. These results indicated that there were abundant genetic variations in this ostrich group，the genetic diversity was very high. |
| keywords:Ostrich Microsatellite marker Genetic diversity Polymorphism information content |
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