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一株韭菜内生高效氯氰菊酯降解生防细菌的分离与鉴定
投稿时间:2009-08-12  修订日期:2009-10-27  点此下载全文
引用本文:任 明,赵 蕾.一株韭菜内生高效氯氰菊酯降解生防细菌的分离与鉴定[J].西北农业学报,2010,19(3):57~61
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作者单位
任 明,赵 蕾 (山东师范大学 生命科学学院济南 250014) 
基金项目:“十一五”国家科技支撑计划重点项目(2006BAD17B00)。
中文摘要:从新鲜韭菜的根中分离到一株对多种蔬菜病原真菌有抑制作用的优势内生细菌W7,对其拮抗机制的初步研究结果表明,胞外代谢物对病原真菌无抑制作用,而菌体经超声波破碎及有机溶剂沉淀得到的多糖粗提液可明显抑制病原菌菌丝生长。该菌还能以100 mg/L高效氯氰菊酯为唯一碳源生长,7 d的降解率为51.3%,表明该菌是一株兼具农药降解特性的生防内生细菌。通过对其形态特征、生理生化及16S rDNA同源性序列分析,初步鉴定该菌为类芽孢杆菌(Paenibacillus spp.)。
中文关键词:内生细菌  拮抗  菌体多糖  高效氯氰菊酯  类芽孢杆菌
 
Isolation and Identification of a Beta cypermethrin degrading and Biocontrol Endophytic Bacteria in Leek
Abstract:One dominant endophytic bacterial strain W7 was isolated from the root of fresh leek,which had a broad inhibition spectrum against several vegetable pathogenic fungi. The results of its antagonistic mechanism showed that the extracellular metabolites had no effect on pathogenic fungi,but the somatic polysaccharose which obtained from cells by ultrasonic disruption and organic solvent precipitation could inhibit the growth of mycelium. Strain W7 was also found to be capable of utilizing beta-cypermethrin as the sole source of carbon for growth,and its degradation within 7 days at initial concentration of 100 mg/L was 51.3%. The results indicated that strain W7 was a pesticide-degrading and an endophytic bacteria suitable for biocontrol. It was identified as Paenibacillus spp. based on morphological,physiological-biochemical properties and 16S rDNA sequence analysis.
keywords:Endophytic bacteria  Antagonism  Somatic polysaccharide  Beta-cypermethrin  Paenibacillus spp.
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