| Hi5-SF悬浮细胞系的建立及鉴定 |
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| 引用本文：马 伟，王家敏，马桂兰，马 祺，马 花，乔自林，马忠仁，冯玉萍.Hi5-SF悬浮细胞系的建立及鉴定[J].西北农业学报,2017,(2):173~178 |
| DOI:10.7606/j.issn.1004-1389.2017.02.003 |
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| 基金项目:教育部“长江学者和创新团队发展计划” (IRT13091)；甘肃省科技计划（144FKCA082）；西北民族大学中央高校基本科研业务费专项资金（31920150029，zyp2015006）。 |
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| 中文摘要:从中国典型培养物保藏中心（CCTCC）引进High Five细胞并对其进行驯化，获得无血清悬浮驯化株（Hi5-SF）。依据《中华人民共和国药典(2010年版三部)》对其进行细胞生物学特性研究、微生物污染、病毒外源因子及细胞致瘤性检测。结果发现，Hi5-SF细胞复苏活力76.3%，生长曲线呈“S”型，最大增殖密度可达3.50×106 mL-1，群体倍增时间为26.2 h；细菌、真菌及支原体检查均为阴性；血细胞吸附试验、致细胞病变试验和特异性病毒荧光抗体结合物检查结果均为阴性；致瘤性检查结果也为阴性。说明该细胞株符合兽用疫苗生产用细胞质量要求，可以在疫苗研究与生产中应用。 |
| 中文关键词:High five细胞 悬浮培养 质量评价 |
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| Establishment and Identification of Hi5-SF Suspension Cell Line |
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| Abstract:High Five cells were introduced from CCTCC and domestecated,,and we obtained semi-adherent cells to suspension growth cell line (Hi5-SF).Subsequently,the biological characteristics,microbial contamination,viral exogenous factors and tumorigenicity of Hi5-SF cell line were detected according to the Pharmacopoeia of People’s Republic of China (3rd Edition,2010).The results showed that the recovery vitality of Hi5-SF cells was 76.3%,the growth curve was S-shape,and maximum concentration was 3.50×106 cells·mL-1 with the population doubling time(PDT) of 26.2 h. The cells were free of bacteria,fungi and mycoplasmas;hemadsorption test,cytopathic test,specific virus fluorescent antibody binding test and tumorigenicity test were all negative. The resultus indicated that the Hi5-SF cell line meets the quality requirements of veterinary vaccine production. |
| keywords:High five cell Suspension culture Quality evaluation |
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