| 转C4基因水稻籽粒产量及品质分析 |
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| 引用本文：张边江 ，唐 宁，陈全战，杨 平，王立科.转C4基因水稻籽粒产量及品质分析[J].西北农业学报,2017,(2):210~215 |
| DOI:10.7606/j.issn.1004-1389.2017.02.008 |
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| 基金项目:江苏省自然科学基金（SBK2015040083）；江苏省高校自然科学基金（14KJB210005）。 |
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| 中文摘要:为了分析C4基因导入C3水稻后对其籽粒产量及品质的影响，以日本水稻品种‘Kitaake’（Oryza sativa L. subsp. japonica）及其转玉米PEPC、PPDK、ME基因水稻为材料，测定C4光合酶活性、光合速率、产量以及主要营养成分。结果表明， 转C4基因水稻的4种光合酶活性显著增加，PEPC基因的导入使光合效率显著提高，与对照相比各转基因株系籽粒中水分、灰分和淀粉质量分数无显著差异，而含有PEPC基因株系的蛋白质和脂肪质量分数显著增加，可溶性糖质量分数降低。可见，PEPC基因的导入加强了籽粒中蛋白质和脂肪的积累，从而使转基因水稻千粒质量和单株穗数显著提高，这可能是转基因水稻光合生产力提高的生理生化基础。 |
| 中文关键词:水稻 C4基因 籽粒产量 营养成分 |
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| Grain Yield and Quality Analysis of Transgenic Rice Expressing C4 Enzymes |
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| Abstract:Taking untransformed rice (Oryza sativa L. subsp.japonica) and transgenic rice with different C4 photosynthesis genes as materials,the activity of C4 photosynthesis enzymes and the net photosynthetic rate (Pn),the moisture,ash,fat,starch,protein and total sugar mass fraction were measured. The results showed that C4 photosynthesis enzymes activities in different transgenic rice had high expression and Pn of PEPC transgenic rice increased significantly. The moisture,ash and starch mass fraction in the transgenic rice were similar to that of control. The protein and fat mass fraction significantly increased in PEPC transgenic rice,the PEPC,PPDK transgenic rice and the transgenic rice with PEPC,PPDK and ME genes,while the soluble sugar mass fraction was significantly lower than those of the control The PEPC gene was beneficial to protein and fat accumulation in transgenic rice,and it maybe the physiological and biochemical basis for improving photosynthetic productivity in transgenic rice expressing C4 enzymes. |
| keywords:Transgenic rice C4 genes Grain yield and quality Nutritional components |
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