| 基于真空渗入法的转基因大白菜植株的获得 |
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| 引用本文：赵 静，吴俊清，李庆飞，张 静，张鲁刚.基于真空渗入法的转基因大白菜植株的获得[J].西北农业学报,2017,(2):248~254 |
| DOI:10.7606/j.issn.1004-1389.2017.02.013 |
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| 基金项目:国家科技支撑计划（2012BAD02B015）；西北农林科技大学唐仲英育种基金；陕西省蔬菜产业体系。 |
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| 中文摘要:采用真空渗入法，将萝卜 VPE1基因的干扰载体pRNAi- RsVPE1转入大白菜中，共收获2 414粒种子，在质量浓度为30 mg/L的卡那霉素筛选下获得抗性株297株，其中9株经PCR检测呈阳性，转化率为0.37%。Real-time PCR检测结果表明，pRNAi- RsVPE1的转入导致转基因大白菜叶片中同源的BraVPE在RNA水平上的表达量下降，验证了转基因植株的可靠性，为进一步深入研究BraVPE基因功能奠定了基础。 |
| 中文关键词:真空渗入法 大白菜 BraVPE转基因 RNAi载体 |
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| Obtaining Transgenic Plants of Chinese Cabbage Based on Vacuum Infiltration |
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| Abstract:The vector pRNAi- RsVPE1 was transformed into Chinese cabbage by vacuum infiltration method and total 2 414 seeds were obtained. The kanamycin concentration 30 mg/L was determined to be the most suitable for selecting transgenic plants. 297 transgenic plants resistant to kanamycin were obtained,of which 9 positive transgenic plants were verified by PCR detection,and the transgenic rate was 0.37%. RT-PCR detection showed that the expression level of BraVPE in transgenic plants decreased significantly,reliability of transgenic plants was verified. Therefore ,the above results laid a foundation for further study in BraVPE gene functions. |
| keywords:Vacuum infiltration Chinese cabbage BraVPE Genetic transformation RNAi vector |
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