Abstract:Tau has good oxidation resistance.In order to investigate the effect of compatibility of Tau and antibiotic on boar semen stored at room temperature, Modena without antibiotic as control,three groups were designed,Modena with gentamicin(2×10 IU) and Cotrimoxazole(2.5×10 IU) as group1, Modena with Tau (0.5 mmol/L,1 mmol/L,5 mmol/L,10 mmol/L) as group 2, and the group3 was diluted with Modena containing both Tau and antibiotic, and sperm motility, acrosome integrity, total antioxidative capacity (T-AOC)activity and malondialdehyde (MDA) content were measured and analyzed.The result showed that Modena supplemented with 5 mmol/L Tau could improve boar sperm motility, effective survival time and acrosome integrity, decrease MDA content and T-AOC activity.The results showed that group 3 was the best, group 2 comes second, and group 1 the worst, the effect had significant difference among them.After 5 days preservation, the group 3 showed that sperm motility, acrosome integrity were 64.11, 72.10%, respectively, T-AOC activity and MDA content were 4.76 U/mL and 1.46 nmol/mL, respectively.In conclusion, Modena supplemented with Tau and antibiotic reduced the oxidative stress and improved the sperm quality of boar semen during liquid storage at normal temperature, and 5 mmol/L Tau was the optimum concentration. |