| 甘蓝型油菜EMS突变后代材料的分子遗传多样性分析 |
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| 引用本文：袁润勤，曲高平，郭 媛，胡胜武.甘蓝型油菜EMS突变后代材料的分子遗传多样性分析[J].西北农业学报,2017,26(6):855~864 |
| DOI:10.7606/j.issn.1004-1389.2017.06.008 |
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| 基金项目:现代农业产业技术体系建设专项资金（CARS-13）。 |
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| 中文摘要:旨在利用SSR和SRAP标记从分子水平上分析甘蓝型油菜EMS突变后代材料的遗传差异。选取138个‘中双9号’油菜的EMS突变M3株系（以‘中双9号’为参照），这些材料的叶、花、角果等存在丰富的形态学变异，选用24对扩增条带清晰、多态性高的SRAP引物和20对SSR引物对参试的139个材料进行分析。结果显示，24对SRAP引物共检测到360个位点，其中102个为多态性位点，平均多态性比率为28.33%，平均多态性信息含量PIC为0.60；20对SSR引物共检测到196个位点，其中91个为多态性位点，平均多态性比率为46.43%，PIC为0.59。参试材料之间的遗传相似系数为0.62~0.91，聚类分析和群体结构分析结果显示，参试EMS突变M3株系没有完全按表型突变聚在一起。研究表明，EMS诱导‘中双9号’产生了分子水平上可检测的突变且诱变后代材料之间存在广泛的遗传差异。 |
| 中文关键词:甘蓝型油菜 EMS突变后代材料 遗传多样性 SSR标记 SRAP标记 |
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| Molecular Genetic Diversity in EMS-induced Mutant Progenies in Brassica napus L. |
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| Abstract:This study was designed to analyze genetic diversity of EMS-induced mutant progenies in Brassica napus L. using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) molecular markers. The selected 138 M3 mutant strains of B.napus cv.‘Zhongshuang No.9’ which served as the control，with abundant morphological variation in leaf，flower and silique organs, was analyzed by 24 SRAP and 20 SSR primer pairs. A total of 360 amplified fragments including 102 polymorphic fragments were detected by 24 SRAP primer combinations，with an average 28.33% of polymorphic fragment percentage and 0.60 of polymorphism information content (PIC) value；whereas，196 amplified fragments containing 91 polymorphic fragments were detected by 20 SSR primer pairs，with an average 46.43% of polymorphic fragment percentage and 0.59 of PIC value. The genetic similarity coefficient between 139 experimental materials varied from 0.62 to 0.91. The cluster and population structure analysis exhibited that experimental materials are not exactly classified depending on phenotype mutation. In general，EMS-induced mutations in ‘Zhongshuang No.9’ can be detected at the molecular level and there are extensive genetic differences among the mutant progenies. |
| keywords:Brassica napus L. EMS-induced mutant Genetic diversity SSR marker SRAP marker |
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