| Abstract:We had respectively analyzed mouse testis tissues of middle age (180-day-old) and old age(360-day-old)by transcriptome sequencing technique. The results showed that 25 187 980 and 28 840 576 reads were obtained in the middle age and old age,and 74.88% and 77.51% reads could be compared to the mouse reference genome. In addition,17 770 genes were found in testis from middle age mouse,and 18 073 genes were detected in old age. As the middle age mouse for reference,185 up-regulated genes and 122 down-regulated genes were detected in old age mouse,and 285 genes were annotated. Through GO analysis,a total of 285 genes were divided into 25 function categories,which involved membrane,transporter activity,oxidation-reduction process,extracellular region,reproductive development and aging et al. KEGG analysis showed different expression genes were annotated to 139 single pathways,including Signal transduction,lipid metabolism,Carbohydrate metabolism,Endocrine system and nervous system. In these differentially expressed genes,the RPKM values of 57 differentially expressed genes were greater than 1,and their corresponding signaling pathways included TNF signaling pathway,complement system,insulin and lysosome-related signaling pathways. In summary,the transcriptome analysis of the testis tissue in middle age and old age mouse revealed the differentially expressed genes,which provides the transcriptome information of middle and old age to enrich the transcriptome datas of mouse testis. |
