Abstract:To investigate the effect of compound ginsenoside and levamisole hydrochloride nanoemulsion (GS-LH-NE) on the immune effect of antigen, mice were divided into normal control group, OVA control group, blank nanoemulsion group, levamisole hydrochloride nanoemulsion (LH-NE) group, ginsenoside nanoemulsion (GS-NE) group, ginsenoside-levamisole hydrochloride (GS-LH) aqueous solution group, GS-LH-NE group (high dosage, medium dosage and low dosage). Mice were subcutaneously injected with antigen OVA twice and intragastrically administrated with drug for three days before the first and second injection. The levels of serum antibodies (IgG, IgG1, IgG2a), the levels of splenocyte cytokines (IFN-γ, IL-4) and proliferation stimulating index of splenic lymphocytes were measured at the 14th day after the second injection. The results showed that the levels of IgG, IgG1 and IL-4 in GS-LH-NE medium dosage (50 mg·kg-1) group were significantly and extremely significantly higher than those in GS-LH-NE low dosage group and LH-NE group. The levels of IgG2a, IFN-γ and proliferation stimulating index of splenic lymphocytes induced by OVA in GS-LH-NE medium dosage (50 mg·kg-1) group were significantly and extremely significantly higher than those in GS-NE group, GS-LH aqueous solution group, LH-NE group, GS-LH-NE high dosage group and low dosage group. Oral administration of GS-LH-NE could induce mixed Th1/Th2 immune response in OVA-inoculated mice, indicating that ginsenoside and levamisole hydrochloride could synergistically enhance the cellular and humoral immune functions in mice. |