| 饲粮中2-羟基-4-甲硫基丁酸异丙酯添加水平对哺乳期山羊生长性能及氨基酸代谢的影响 |
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| 引用本文：李录明，张恩平，高亚伟，李金朋.饲粮中2-羟基-4-甲硫基丁酸异丙酯添加水平对哺乳期山羊生长性能及氨基酸代谢的影响[J].西北农业学报,2018,27(12):1745~1753 |
| DOI:10.7606/j.issn.1004-1389.2018.12.006 |
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| 基金项目:陕西省重点研发计划项目（201TSCXL-NY-04-02）；陕西省农业科技创新转化项目（NYKJ-2016-04）。 |
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| 中文摘要:旨在研究饲粮中2-羟基-4-甲硫基丁酸异丙酯（HMBi）添加水平对哺乳期山羊生长性能及氨基酸代谢的影响，为HMBi在山羊日粮中的添加应用提供理论参考。将27只健康、体质量相近（49.79±1.86 kg）且均产单羔的哺乳期陕北白绒山羊随机分为3组，对照组饲喂基础日粮，试验组在基础日粮中分别添加2% 和4% 的HMBi，试验期为60 d。测定各组羊试验期平均日增加体质量（ADG）、乳成分、血液生化指标、血浆游离氨基酸质量浓度和乳水解氨基酸质量分数。结果表明：添加HMBi能够减轻哺乳期母羊体质量损失，2% 组山羊哺乳期ADG显著高于对照组（P＜0.05）。对照组羊乳脂质量分数显著低于2% 组（P＜0.05），极显著低于4% 组（P＜0.01）；对照组羊乳糖质量分数显著高于2% 组（P＜0.05），显著低于4% 组（P＜0.05）；对照组羊乳蛋白、乳中总固形物和乳中非脂固形物质量分数均极显著低于2% 组和4% 组（P＜0.01）；2% 组羊乳蛋白和乳中非脂固形物质量分数极显著高于4% 组（P＜0.01），乳糖和乳中总固形物质量分数极显著低于4% 组（P＜0.01）。试验第60天，4%组山羊血清尿素氮（BUN）浓度显著低于对照组（P＜0.05），血浆Met质量浓度显著高于对照组（P＜0.05）；2% 组山羊血浆Thr、Val和Leu质量浓度显著高于4% 组和对照组（P＜0.05），血浆Lys质量浓度极显著高于对照组（P＜0.01）。试验山羊乳中Met、必需氨基酸和总氨基酸质量分数随HMBi添加水平的提高有增加趋势，但各组之间差异不显著（P＞0.05）。综上所述，哺乳期山羊饲粮中添加HMBi能够减轻山羊哺乳期体质量损失，改善山羊血清生化指标，提高山羊乳成分质量分数和血浆Met质量浓度；哺乳期山羊饲粮中2% HMBi的添加效果优于4%。 |
| 中文关键词:山羊 2-羟基-4-甲硫基丁酸异丙酯 生长性能 氨基酸代谢。 |
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| Effects of Supplementation with 2-hydroxy-4-(methylthio) Butanoic Acid Isopropyl Ester on Growth Performance and Amino Acids Metabolism in Lactating Goats |
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| Abstract:This experiment was aimed to investigate the effects of supplementation with 2-hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMBi) on growth performance and amino acids metabolism in lactating goats, and to provide a reference for the use of HMBi on goats. 27 healthy lactating Shaanbei White Cashmere goats with similar body mass (49.79±1.86 kg) were selected and randomly assigned to 3 groups. The control group was fed the basal diets, the test groups was fed the basal diets and supplemented 2% and 4% HMBi respectively The experiment lasted for 60 days. Average daily gain, milk composition, serum biochemistry indices, plasma free amino acid mass concentration and milk hydrolytic amino acid mass fraction were measured. The results showed that:the average daily gain of goats in 2% group was significantly higher than those in control group (P＜0.05).The milk fat mass fraction of goats in control group were significantly lower than those in 2% group (P＜0.05), and significantly lower than those in 4% group (P＜0.01);the milk lactose mass fraction of goats in control group were significantly higher than those in 2% group (P＜0.05), and significantly lower than those in 4% group (P＜0.05); the milk protein, milk total solid content and milk solid not fat mass fraction of goats in control group were significantly higher than those in 2% group and 4% group (P＜0.01);the milk protein and milk solid not fat mass fraction of goats in 2% group were significantly higher than those in 4% group (P＜0.01), the milk lactose and milk total solid mass fraction were significantly lower than those in 4% group (P＜0.01).On 60 d, the serum urea nitrogen concentration of goats in 4% group was significantly lower than those in control group（P＜0.05), plasma Met mass concentration of goats in 4% group was significantly higher than those in control group (P＜0.05), plasma amino acids mass concentration of Thr, Val and Leu in 2% group was significantly higher than those in control group and 4% group (P＜0.05), plasma Lys mass concentration of goats in 2% group was extremely significantly higher than those in control group (P＜0.01).It showed increasing trend on mass fraction of milk Met and total amino acids with the addition of HMBi level. This study concluded that HMBi supplementation in diet could reduce the body mass lose of lactating goats, and improve serum biochemical indices, and increase the mass concentration of plasma Met; the 2% HMBi supplement level in diet had better effects on lactating goats. |
| keywords:Goat 2-Hydroxy-4-(methylthio) butanoic acid isopropyl ester (HMBi ) Growth performance Amino acid metabolism. |
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