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一个玉米叶夹角突变体的表型鉴定及遗传分析
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引用本文:王晓娟,何海军,刘忠祥,杨彦忠,寇思荣,连晓荣,周玉乾,周文期.一个玉米叶夹角突变体的表型鉴定及遗传分析[J].西北农业学报,2019,28(8):1226~1231
DOI:10.7606/j.issn.1004-1389.2019.08.003
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作者单位
王晓娟,何海军,刘忠祥,杨彦忠,寇思荣,连晓荣,周玉乾,周文期 (甘肃省农业科学院 作物研究所兰州 730070) 
基金项目:国家自然科学基金(31860383);国家重点研发计划(2018YFD0100202-3);甘肃省玉米产业技术体系(2018GARS-02-03)。
中文摘要:玉米叶夹角是高密度育种的重要影响因子,与株型育种及产量高度相关;叶夹角相关QTL/基因的鉴定不仅有助于剖析叶夹角的遗传基础,也为玉米叶夹角及株型的遗传改良提供重要的分子靶点。以自交系‘LY8405’自然突变获得的突变体‘FU1603’为主要研究材料,开展叶夹角性状的表型鉴定、遗传分析及定位等试验。结果表明,与‘LY8405’相比,‘FU1603’穗三叶的叶夹角显著降低(P<0.05),而且伴随有叶片卷曲等表型;且在植株的株型、穗部性状及籽粒性状上均发生了显著的改变(P<0.05)。遗传分析表明‘FU1603’为一个单隐性核基因控制的突变体(χ值>0.5)。采用BSA (Bulked segregant analysis)方法筛选到与突变位点紧密连锁的3个SSR标记C1-2、C1-16和C1-18,利用‘FU1603’与‘B73’自交系杂交产生的160个F2单株开展了上述3个连锁标记的共分离分析;进一步利用此3个标记筛选后代重组交换单株,最终将控制叶夹角的突变位点定位在玉米第一染色体1.02 bin标记C1-18和C1-2之间9 Mb范围之内,为后续该位点的精细定位及克隆奠定良好的基础。
中文关键词:玉米  叶夹角  突变体  BSA  遗传分析  基因定位
 
Phenotyping and Genetic Analysis of Leaf Angle Mutant in Maize (Zea mays L.)
Abstract:Leaf angle is an important trait for high density breeding in maize,which is highly correlated with plant architecture and crop production. So the identification of QTL or genes related to leave angle is not only useful to uncover the genetic mechanism of leaf angle,but also important for practical application value in the high density breeding and architecture breeding. ‘FU1603’,a natural mutant from the maize inbred line ‘LY8405’,was served as the material to identify phenotype,genetic analysis and gene mapping. The results showed that ‘FU1603’ has a phenotype of small leaf angles and curled leaves. The other agronomic traits of the mutant ‘FU1603’ including the plant architectures,ears,tassels and kernels were also changed significantly,compared with ‘LY8405’. The genetic analysis indicated that ‘FU1603’ was a mutant with small leaf angle controlled by a single recessive nuclear gene. Three SSR markers closely linked to this locus were identified by BSA method,namely C1-2,C1-16,C1-18,and all of them located in the 1.02 bin of chromosome 1 in maize. To verify the three linked SSR markers,160 F2 plants were used,and the mutation site was mapped between markers C1-18 and C1-2 with recombinant plants,which was about 9 Mb physical region according to ‘B73’ reference genome.
keywords:Maize  Leave angle  Mutant  Bulked segregant analysis(BSA)  Genetic  Gene Mapping
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