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水分胁迫对赤霞珠葡萄果实花色苷生物合成的影响
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引用本文:吕丹桂,谢 岳,徐伟荣,王振平.水分胁迫对赤霞珠葡萄果实花色苷生物合成的影响[J].西北农业学报,2019,28(8):1274~1281
DOI:10.7606/j.issn.1004-1389.2019.08.009
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作者单位
吕丹桂,谢 岳,徐伟荣,王振平 (宁夏大学 农学院银川 750021) 
基金项目:国家现代农业产业技术体系(CARS-29-zp-03);国家自然科学基金(31660551)。
中文摘要:为研究不同水分胁迫对葡萄果实花色苷生物合成相关基因表达与总花色苷质量分数之间的关系。以3 a生‘赤霞珠’为试材,在果实转色前1周进行水分胁迫处理,测定了采收期葡萄果实百粒质量、可溶性固形物质量分数、可滴定酸质量浓度、总酚和单宁质量分数,用pH示差法和qRT-PCR技术分别检测果实总花色苷质量分数和花色苷合成相关基因的表达量。结果表明:轻度和中度胁迫降低了采收期果实百粒质量和可滴定酸质量浓度,增加了可溶性固形物质量分数、单宁和总酚质量分数。同时,轻度和中度胁迫也提高了果实成熟过程中总花色苷质量分数,在花后110 d达到最大,分别为0.241%和0.228%;轻度和中度胁迫上调了PAL CHS1DFR F3′H F3′5′HUFGT MybA1基因的表达量,其中花后90 d, CHS1 F3′H F3′5′H MybA1基因的表达量最高;花后100 d,PALDFR基因的表达量最高;花后110 d,UFGT基因的表达量最高。重度水分胁迫会降低果实成熟后期 CHS1 F3′H F3′5′HUFGT表达量。相关性分析表明,中度水分胁迫 CHS1表达量与总花色苷质量分数呈显著正相关,重度水分胁迫PAL MybA1基因表达量与总花色苷质量分数呈显著正相关。轻度和中度水分胁迫促进葡萄花色苷生物合成中相关基因的表达,从而提高葡萄果实总花色苷质量分数。
中文关键词:水分胁迫  葡萄果实  花色苷  基因
 
Effect of Water Stress on Anthocyanin Biosynthesis of Grape Berries
Abstract:To investigatethe effect of water stress on the relationship between anthocyanin biosynthesis related gene expression and total anthocyanin mass fraction in grape berries,three-year-old Cabernet Sauvignon was selected as experimental material treated with differentwater stress at one week before veraison.The hundred grain mass,total soluble solids, titratable acids mass concentration,total phenols and tannins mass fraction of grape berries during harvest were determined.The mass fraction of total anthocyanins and the expression of genes related to anthocyanin biosynthesis in grape berries during ripening were detected by pH differential method and RT-PCR, respectively.The results showed that mild and moderate stress reduced hundred grain quality and titratable acid mass fraction, increased total soluble solid mass fraction,tannin and total phenol mass fraction.Meanwhile, mild and moderate stress also increased the total anthocyanin mass fraction during fruit ripening, reaching the maximum,0.241% and 0.228% respectively,at 110 days after anthesis.Mild and moderate water stress increased the expression of PAL, CHS1, DFR, F3′H, F3′5′H, UFGT and MybA1 genes in anthocyanin biosynthesis pathway.Among them, CHS1, F3′H, F3′5′H and MybA1 genes expressed the highest at 90 days after anthesis; PAL and DFR genes expressed the highest at 100 days after anthesis; UFGT genes expressed the highest at 110 days after anthesis.While severe water stress decreased the expression of CHS1, F3′H, F3′5′H and UFGT at late ripening stage.Correlation analysis showed that there was a significant positive correlation between the total anthocyanin mass fraction in moderate water stress and the expression of CHS1 gene.The total anthocyanin mass fraction in severe water stress also were positively correlated with the expression levels of PAL and MybA1 genes.Mild and moderate water stress promoted the expression of related genes in grape anthocyanin biosynthesis, thus increasing the mass fraction of total anthocyanin in grape berries.
keywords:Water stress  Grape berry  Anthocyanin  Gene
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