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超表达枳β-淀粉酶基因( PtrBAM1)对烟草不同糖和有机酸的影响
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引用本文:肖 畅,彭 婷.超表达枳β-淀粉酶基因( PtrBAM1)对烟草不同糖和有机酸的影响[J].西北农业学报,2019,28(12):2005~2010
DOI:10.7606/j.issn.1004-1389.2019.12.012
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作者单位
肖 畅,彭 婷 (1.湖北广播电视大学 导学中心武汉 430000
2.赣南师范大学 生命科学学院江西赣州 341000) 
基金项目:江西省自然科学基金(20171BAB204024)。
中文摘要:利用气相色谱法测定低温处理下3个 PtrBAM1超表达烟草株系(F6、F11、F25)与两个对照组(空载1301和野生型WT)烟草中可溶性糖和有机酸的含量,旨在从改变植株糖酸含量的角度解析 PtrBAM1的抗寒功能。结果表明:正常条件下超表达株系的β-淀粉酶活性及果糖、蔗糖、柠檬酸、苹果酸和奎宁酸含量均显著高于对照,2 ℃低温处理6 h、1 d、3 d后超表达株系的β-淀粉酶活性、果糖含量均显著高于2个对照组。F6、F11、F25葡萄糖含量在6 h~1 d时有一个骤升的过程,而柠檬酸含量一直显著高于1301和WT,苹果酸和奎宁酸含量除1 d外,其他时间点含量均显著高于对照。由此可见, PtrBAM1在低温处理下可能通过改变糖酸代谢途径增强植株的抗寒性。
中文关键词:烟草  柑橘  低温  葡萄糖  果糖  蔗糖  苹果酸  柠檬酸  奎宁酸
 
Overexpressing a β-Amylase Gene, PtrBAM1,Changes the Contents of Soluble Sugars and Organic Acids in Tobacco
Abstract:Three PtrBAM1-overexpressing tobacco lines were created in our previous studies: F6,F11,F25. In the current experiment,the contents of different sugars and organic acids in cold-treated F6,F11,F25,1301 (the empty vector line) and nud (the wild type) were analyzed via GC-MS. Results showed that,under normal condition,β-amylase activity,the contents of fructose,sucrose,citric acid,malic acid and quinine acid in F6,F11,and F25 lines were significantly higher than those of 1301 and nud. After 2 ℃ treatment for 6 h,1 d and 3 d,β-amylase activity and the content of fructose in the three overexpressing lines were significantly higher than those of the 1301 and nud lines. The content of glucose increased sharply from 6 h-1 d cold treatment. Throughout the 2 ℃ treatment,the content of citric acid in F6,F11,and F25 lines were constantly and significantly higher than that of 1301 and nud lines,so were the content of malic acid and quinic acid except at the timepoint of 1 d. In conclusion, PtrBAM1 could change the contents of soluble sugars and organic acids in cold- challenged plant,thus increasing the cold tolerance of plant,at least in part,via modification of the sugar and organic acid metabolism.
keywords:Tobacco  Citrus  Low temperature  Glucose  Fructose  Sucrose  Malic acid  Citric acid  Quinic acid
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