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新疆马铃薯镰刀菌根腐病发生危害调查及病原菌鉴定
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引用本文:杨 波,郭成瑾,王喜刚,沈瑞清.新疆马铃薯镰刀菌根腐病发生危害调查及病原菌鉴定[J].西北农业学报,2019,28(12):2069~2077
DOI:10.7606/j.issn.1004-1389.2019.12.020
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作者单位
杨 波,郭成瑾,王喜刚,沈瑞清 (1.宁夏大学 农学院银川 7500212.宁夏农林科学院 植物保护研究所银川 750002) 
基金项目:国家公益性行业(农业)科研专项(201503112+7);宁夏农林科学院全产业链创新示范项目(NKYZ16-0104)。
中文摘要:为明确新疆马铃薯镰刀菌根腐病的发生危害及病原菌的种类,通过对新疆地区马铃薯镰刀菌根腐病害进行调查,对病原菌进行形态学和分子生物学鉴定,然后依据柯赫氏法则进行病原菌确认。结果表明,不同马铃薯种植地区病害发生率及严重程度存在一定差异,其中病害发生最高的地区是伊宁县,病害发生率达80%。经鉴定,引起新疆地区马铃薯镰刀菌根腐病的病原菌有尖孢镰刀菌、木贼镰刀菌、芬芳镰刀菌、茄病镰刀菌和锐顶镰刀菌。通过致病性测定发现,鉴定出的5种镰刀菌均具致病性,其中茄病镰刀菌为新疆马铃薯镰刀菌根腐病的优势致病菌,致病率达88.9%;其次是芬芳镰刀菌,致病率为66.7%。
中文关键词:马铃薯  镰刀菌  形态学鉴定  分子生物学鉴定
 
Investigation and Pathogen Identification of Potato Root Rot Caused by Fusarium in Xinjiang
Abstract:In order to clarify the occurrence and types of potato root rot caused by Fusarium in Xinjiang, the morphological and molecular biological identification of the pathogen was carried out through the investigation of potato root rot by Fusarium in Xinjiang, and then the pathogen was confirmed according to Koch’s rule.The results showed that there were some differences in the incidence and severity of diseases in different potato planting areas, among of them,the highest incidence, 80%, was detected in Yining county. The causal Fusarium pathogens related to potato root rot in Xinjiang were detected, including F.oxysporum, F.equiseti, F.redolens, F.solani and F.acuminatum. These five Fusarium strains were all pathogenic, andthe dominant pathogen in Xinjiang was F.solani with the pathogenicity of 88.9%, followed by F.redolens with the pathogenicity of 66.7%.
keywords:Potato  Fusarium  Morphological identification  Molecular identification
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