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盐胁迫下 OsDSR2 RNAi转基因水稻的主要农艺性状分析
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引用本文:楚乐乐,罗成科,路旭平,李芳兰,马天利,李培富.盐胁迫下 OsDSR2 RNAi转基因水稻的主要农艺性状分析[J].西北农业学报,2020,30(6):877~885
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作者单位
楚乐乐,罗成科,路旭平,李芳兰,马天利,李培富 (宁夏大学 农学院宁夏优势特色作物现代分子育种重点实验室银川 750021) 
基金项目:国家自然科学基金(31560297)。
中文摘要:为探究盐胁迫下 OsDSR2 抑制表达后对水稻主要农艺性状和产量的影响,进一步阐明 OsDSR2 参与调控水稻耐盐机制,以野生型植株中花11(ZH11)和 OsDSR2 RNAi转基因水稻不同株系DR14和DR20为试验材料,植株幼穗分化期进行0.15 mol/L盐胁迫处理,待植株完熟后测定穗长、每穗总粒数、每穗实粒数、结实率、单株穗数、单株粒质量、千粒质量等主要农艺性状,并进行相关性和主成分分析。结果表明,盐胁迫后 OsDSR2 RNAi转基因水稻的单株穗数、结实率均显著高于野生型ZH11,正常条件下, OsDSR2 RNAi转基因水稻单株粒质量均显著低于野生型ZH11,盐胁迫后二者均降低但无显著性差异。与正常条件下相比,盐胁迫处理后 OsDSR2 RNAi转基因植株的千粒质量与每穗实粒数呈显著负相关性,单株粒质量与穗长、结实率、千粒质量呈负相关性,单株穗数与穗长、结实率呈负相关性,单株粒质量与每穗总粒数呈正相关性;结实率与穗长、每穗总粒数呈负相关性。盐胁迫处理后 OsDSR2 RNAi转基因水稻第一、二主成分中,分别是单株粒质量和结实率特征向量最大。综上,盐胁迫下 OsDSR2 的抑制表达主要通过抑制水稻植株的单株穗数、结实率和单株粒质量的降低,协调单株粒质量与单株穗数、结实率、千粒质量的关系以及结实率与千粒质量、单株穗数的关系,从而调控盐胁迫下水稻的产量。
中文关键词:盐胁迫   OsDSR2 ;水稻;农艺性状
 
Main Agronomic Traits Analysis of OsDSR2 RNAi Transgenic Rice under Salt Stress
Abstract:In order to explore the effect of OsDSR2 inhibition under salt stress on the main agronomic traits and yield of rice, and clarify the mechanism of OsDSR2 involved in the regulation of rice salt tolerance.In this experiment, Zhonghua 11(ZH11) in wild-type plants and OsDSR2 RNAi transgenic rice was used as experimental material.Salt stress of 0.15 mol/L was applied at young panicle differentiation stage of rice, and the main agronomic traits including panicle length,total grains per panicle,filled grains per panicle,filled grains rate,panicle per plant,grain mass per plant,1 000-grain mass were determined after the plants were fully grown, and the correlation and principal component analysis were performed.The results showed that panicle per plant and filled grain rate per panicle of OsDSR2 RNAi transgenic rice were significantly higher than that of wild-type ZH11 after salt stress. Under normal condition, the grain mass per plant of OsDSR2 RNAi transgenic rice was significantly lower than that of wild-type ZH11, its grain mass per plant decreased but it had no significant difference after salt stress. Compared with the normal condition,the 1 000-grain mass had significantly negative correlation with filled grains per panicle of OsDSR2 RNAi transgenic rice after salt stress, and grain mass per plant had a negative correlation with panicle length,filled grains rate and 1 000-grain mass .The grain mass per plant had positive correlation with total grains. The panicle per panicle had negative correlation with panicle length and filled grains rate per panicle. The filled grains rate per panicle had negative correlation with panicle length and total grains per panicle. Among the first and second principal components of OsDSR2 RNAi rice, the eigenvectors of panicle length and grain mass were the greatest. In conclusion, the inhibition expression of OsDSR2 increased grain mass per plant and impact of grain rate per panicle mainly by affecting the panicle per plant, filled grains rate and decrease of grain mass per plant, and by coordinating the relationships among the grain mass per plant, panicle per plant, grain yield per panicle and 1 000-grain mass per panicle as well as the relationships among filled grains,1 000-grain mass and panicle per plant.
keywords:Salt stress   OsDSR2   Rice  Agronomic traits
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