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中药提取物对IBDV感染SPF鸡的防治效果
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引用本文:朱买勋,唐红梅,闫志强,陈春林,翟少钦.中药提取物对IBDV感染SPF鸡的防治效果[J].西北农业学报,2021,(1):25~31
DOI:10.7606/j.issn.1004-1389.2021.01.004
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作者单位
朱买勋,唐红梅,闫志强,陈春林,翟少钦 (重庆市畜牧科学院重庆 402460) 
基金项目:重庆荣昌农牧高新技术产业研发专项(cstc2019ngzx0003)。
中文摘要:为了确定中药提取物对IBDV感染的防治效果,将150羽SPF雏鸡随机分为对照组、模型组和中药提取物高、中、低剂量组,中药提取物高、中、低剂量组分别在饮水中添加10.0、5.0、2.5 g/L的中药提取物,连续添加7 d,对照组和模型组不添加任何药物。给药后,除对照组外,其余组雏鸡分别通过滴鼻接种IBDV,攻毒后连续观察2周。统计雏鸡的成活率,荧光定量PCR检测IBDV在组织中的载量,分析病毒在脏器中的分布。结果显示:中药提取物能提高雏鸡成活率,高、中、低剂量组雏鸡成活率分别为86.67%、93.33%和 70.00%,高剂量组雏鸡脾脏和法氏囊指数显著降低,中剂量组的胸腺和法氏囊指数显著降低。高、中剂量组脾脏、胸腺、法氏囊病毒载量极显著低于模型组,脾脏、胸腺病毒载量极显著低于低剂量组,法氏囊病毒载量显著低于低剂量组。表明中药提取物能提高雏鸡的成活率,促进IBDV核酸转阴,改善脾脏、胸腺、法氏囊等免疫器官状况,降低病毒在脾脏、法氏囊、胸腺的载量,且中剂量组效果优于其他剂量组。
中文关键词:SPF雏鸡  IBDV  中药提取物  抗病能力  病毒载量
 
Preventive Effect of Traditional Chinese Medicine Extractive on SPF Chicks Inoculated with IBDV
Abstract:In order to investigate the preventive effect of traditional Chinese medicine extractive (TCME) on SPF chicks inoculated with IBDV. 150 SPF chicks were randomly divided into control group,model group,TCME high,medium and low dose groups. In groups of TCME high,medium and low dose,extractive doses of 10.0,5.0,2.5 g/L were respectively added into drinking water for 7 consecutive day, but no drug was added in the control group and model group.Except for control group,the experimental chicks in the other groups were inoculated with IBDV by nasal intubation and were continuously observed for 2 weeks,meanwhile,the survival rate was statistically analyzed. The IBDV load was detected for analyzing the distribution of virus in tissues by RT-qPCR. The results showed that the TCME could improve the survival rate of chicks,the survival rate of chicks in groups of high,medium and low dose reached 86.67%,93.33%,70.00%,respectively. The spleen index and bursal index of chicks inoculated with IBDV in high dose group significantly decreased and thymus index and bursal index of chicks in medium dose group significantly decreased.The IBDV load of spleen,thymus and bursa in high and medium dose group were significantly lower than that in model group,the spleen and thymus were significantly lower than that in low-dose group,bursa were significantly lower than that in low-dose group. These results indicated that TCME could improve the survival rate of chicks and promote IBDV positive to negative,improve the chicks’spleen,thymus and bursal organ in its lower virus load,and the effect of medium dose is superior to the other doses.
keywords:SPF chicks  IBDV  Traditional Chinese medicine extractive  Antiviral ability  Viral load
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