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2株优良芽孢杆菌的筛选鉴定及促生特性
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引用本文:孙一凡,冯朝阳,冯冰聪,刘 喆,郑泽浩,郭 俏,来航线.2株优良芽孢杆菌的筛选鉴定及促生特性[J].西北农业学报,2021,(1):132~142
DOI:10.7606/j.issn.1004-1389.2021.01.016
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作者单位
孙一凡,冯朝阳,冯冰聪,刘 喆,郑泽浩,郭 俏,来航线 (西北农林科技大学 资源环境学院陕西杨凌 712100) 
基金项目:陕西省重点研发计划(2019ZDLNY01-07-01)。
中文摘要:选用实验室保存的10株芽孢杆菌,通过皿内拮抗试验、皿内种子发芽试验、菌株碳氮源利用试验、菌株分子生物学鉴定等手段筛选鉴定出MY1、CY1两株芽孢杆菌,它们具有广谱抑菌性和潜在促生能力,其中MY1为贝莱斯芽孢杆菌(Bacillus velezensis),CY1为枯草芽孢杆菌沙漠亚种(Bacillus subtilis subsp. inaquosorum)。进一步通过盆栽试验研究MY1、CY1对番茄促生及抗性的影响,结果表明MY1、CY1发酵液可显着提高番茄株高、叶片叶绿素含量、地上部分干质量等生物学指标,PAL、PPO、POD、CAT 4种防御酶活性分别在不同测试时间显著高于对照组,表明MY1、CY1具有促进番茄生长,增强植株抗病性的能力。
中文关键词:芽孢杆菌  鉴定  拮抗作用  促进植物生长
 
Screening and Identification of Two Bacillus sp.and Their Growth Promotion Characteristics
Abstract:Ten strains of Bacillus sp.in our laboratory were seleceted as materials,the experiments of in vitro antagonism, tomato seedling growth, carbon and nitrogen source utilization,molecular biological identification of bacterial strains,etc, were conducted,two strains of Bacillus(Bacillus velezensis MY1,Bacillus subtilis subsp. inaquosorum CY1 ) with broad-spectrum bacteriostasis and potential growth-promoting ability were screened out.Furthermore, the effects of MY1 and CY1 on tomato growth promotion and resistance were determined in pot experiments. The results showed that MY1 and CY1 fermentation broth could significantly increase tomato biological parameters such as plant height, leaf chlorophyll content and aboveground dry mass. The activities of the defense enzymes POD, CAT, PAL and PPO were significantly higher than those in the control group at different test times. The result demonstrated that two strains of Bacillus MY1 and CY1 had the ability to promote tomato growth and enhance plant disease resistance.
keywords:Bacillus sp  Identification  Antagonism  Plant growth promotion
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