| 小麦KUP/HAK/KT基因家族的全基因组鉴定、系统进化和表达模式分析 |
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| 引用本文：吴胜男，杨 媛，李英壮，王 娜，谢彦周，简俊涛，杨 辉，王成社.小麦KUP/HAK/KT基因家族的全基因组鉴定、系统进化和表达模式分析[J].西北农业学报,2021,30(3):351~364 |
| DOI:10.7606/j.issn.1004-1389.2021.03.004 |
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| 基金项目:国家重点研发计划（2016YFD0102101）；国家自然科学基金（31101139）。 |
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| 中文摘要:为深入发掘小麦KUP/HAK/KT基因的功能，利用小麦最新基因组数据，通过生物信息学手段，对小麦KUP/HAK/KT基因家族进行基因组水平的鉴定，并对其系统进化及表达模式进行分析。鉴定结果表明，本研究在小麦中鉴定到98个KUP/HAK/KT基因，根据系统进化分析结果，可将其分为ClusterⅠ、ClusterⅡ、ClusterⅢ和ClusterⅣ4个进化簇，不同进化簇具有特异的基因结构。染色体定位结果表明，小麦21条染色体上均含有KUP/HAK/KT基因，每条染色体上有2～9个KUP/HAK/KT基因。通过对小麦KUP/HAK/KT基因复制事件的分析发现小麦KUP/HAK/KT基因共产生92个基因复制。此外，转录组数据分析发现小麦KUP/HAK/KT基因在逆境胁迫下存在差异表达，多个胁迫响应相关的KUP/HAK/KT基因被鉴定到，为小麦KUP/HAK/KT基因功能的研究提供了初步的理论依据。 |
| 中文关键词:小麦 KUP/HAK/KT 系统进化 表达模式 |
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| Genome-wide Identification, Phylogeny and Expression Analysis of KUP/HAK/KT Transcription Factors in Wheat |
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| Abstract:The plant genome contains a large number of HAK/KUP/KT transporters that play an important role in plant growth and development, which includes potassium absorption and transport, osmotic adjustment, salt tolerance, and root morphogenesis. In order to deeply explore the function of wheat KUP/HAK/KT genes, the bioinformatics method was used to identify the KUP/HAK/KT gene family at the genome-wide level of wheat, and to analyze their systematic evolution and expression patterns. In this study, 98 putative KUP/HAK/KT genes were identified and these genes were mapped to 21 chromosomes of wheat. Based on the results of phylogenetic analysis, the 98 genes could be divided into four evolution clusters:Cluster Ⅰ, Cluster Ⅱ, Cluster Ⅲ, and Cluster Ⅳ, and KUP/HAK/KT members in different cluster possessed the specific gene structures. In addition, a total of 92 TaKUP/HAK/KT gene pairs were designated as arising from duplication events. The analysis of RNA-seq data found that the wheat KUP/HAK/KT genes showed differential expression under different stresses, and most TaKUP/HAK/KT genes were identified to be involved in the stress response. These findings laid the foundation for studying KUP/HAK/KT gene function in wheat. |
| keywords:Wheat KUP/HAK/KT Phylogeny Expression pattern |
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