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不同产地的两种橙色大白菜类胡萝卜素积累差异分析
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引用本文:马 帅,王若凡,陈 霖,孟 艳,卢倩倩,张鲁刚.不同产地的两种橙色大白菜类胡萝卜素积累差异分析[J].西北农业学报,2021,(3):395~405
DOI:10.7606/j.issn.1004-1389.2021.03.008
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作者单位
马 帅,王若凡,陈 霖,孟 艳,卢倩倩,张鲁刚 (西北农林科技大学 园艺学院陕西杨凌 712100) 
基金项目:国家重点研发计划(2017YFD0101802)。
中文摘要:为了进一步了解橙色大白菜类胡萝卜素积累与地域之间的关系,以国内8个不同地区生产的橙色大白菜‘14杂1’和‘15杂22’叶球为试验材料,利用高效液相色谱法对其类胡萝卜素进行定性和定量分析。结果表明,不同地区生产的两个橙色大白菜叶球的β-胡萝卜素含量相对稳定,α-胡萝卜素和番茄红素的含量差异显著,且趋势不一致。在所有地区中,‘14杂1’叶球的α-胡萝卜素含量均高于‘15杂22’,并且两个品种的β-胡萝卜素含量均低于α-胡萝卜素。有7个地区的‘14杂1’的番茄红素含量低于‘15杂22’,且含量差异显著,与产地关系密切,以湖北武汉地区生产的橙色大白菜番茄红素的含量最高。荧光定量PCR的方法分析发现两个品种叶球中LYCE表达与α-胡萝卜素积累趋势相一致,番茄红素异构酶基因(CRTISO)的表达与番茄红素的积累趋势相似。试验还表明,橙色大白菜在关键基因番茄红素异构酶突变体(Brcrtiso)存在下,其叶球中类胡萝卜素积累的组分和数量还受遗传背景、环境条件及其互作的综合影响,其机制有待进一步研究。
中文关键词:橙色大白菜  产地  类胡萝卜素  基因表达
 
Analysis of Carotenoid Accumulation Differences between Two Kinds of Chinese Cabbage with Orange Head in Different Production Areas
Abstract:In order to further study the relationship between carotenoid accumulation and environment in Chinese cabbage with orange head,the leaves of Chinese cabbage with orange head ‘14 za 1’ and ‘15 za 22’ from 8 different regions of China were usedas materials in this paper, the qualitative and quantitative analysis of carotenoids were conducted by use of high efficiency liquid chromatography. The results showed that the content of β-carotene in the leaves of the two varieties from different areas are relatively stable, and the contents of α - carotene and lycopene were significantly different, and the trends were not consistent. In all regions, ‘14 za 1’ had a higher content of α-carotene than ‘15 za 22’, and both varieties had lower content of β-carotene than α-carotene. In 7 regions, the content of lycopene in ‘14 za 1’ was lower than that in ‘15 za 22’, and the content was significantly different among regions, which reached the highest level in Wuhan, Hubei province. It was found that the expression of LYCE in the two cultivars was consistent with the accumulation trend of α-carotene, and the expression of crtiso was similar to the accumulation trend of lycopene. The results indicated that the accumulation of carotenoids in Chinese cabbage with orange head is influenced by the genetic background, environmental conditions and their interaction under the presence of the key gene Brcrtiso mutation. The mechanism is needed to further studied.
keywords:Chinese cabbage with orangehead  Production areas  Accumulation of carotenoids  Gene expression
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