Abstract:In order to explore short-term effect of partial substitution of inorganic fertilizer with organic fertilizer on soil fertility and fungal community characteristic,with soil in Qinghai-Tibet Plateau as research object,three fertilization treatments were set,which include conventional chemical fertilizer of 1 500 kg/hm,(XN001CK); combination of 50% chemical fertilizer with organic fertilizer of 45 000 kg/hm(XN002),and 30% chemical fertilizer with organic fertilizer of 60 000 kg/hm(XN003).The changes of soil nutrient content and fungal community structure under different treatments after two years were studied by use of conventional analysis method and Illumina MiSeq high-throughput sequencing technique.Compared with XN001 treatment,the treatment of substitution of chemical fertilizer with organic fertilizer(XN002 and XN003)significantly increased the contents of organic matter,total nitrogen,available phosphorus and available potassium and abundance of fungus communities in soil,but significantly decreased the soil pH.For 13 known fungal phyla in soil,Ascomycota and Basidiomycota were identified as the dominant fungal communities with an average relative abundance,accounting for 47.87% and 4.81%,respectively.Besides,the relative abundance of Ascomycota and Basidiomycota in soil under treatment of XN003 were 7.81% and 12.35% respectively higher than that under treatment of XN001.At the genus level,the fungal community structure in soil was differentwith control treatment after organic fertilizer was increasinglyapplied,of which the relative abundance of beneficial fungi Mortierella increased by 1.86% and the Fusarium associated with crop diseases decreased by 3.87% under the XN003 treatment.The Pearson’s correlation analysis showed that fungal richness indexes,including the Chao1 and ACE,were negatively correlated with pH and positively correlated with organic matter,total nitrogen,available phosphorus,and available potassium in soil.Among them,the content of available phosphorus played a major role in determining variation of fungal diversity in soil.In conclusion,the partial substitution of inorganic fertilizer with organic fertilizer can be used to effectively enrich the soil fertility,optimize the soil fungal community structure,increase the proportion of soil beneficial fungi and decrease the relative abundance of soil pathogenic fungi in the short-term. |