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连作对马铃薯酚酸类自毒物质及根际真菌群落的影响
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引用本文:龚 静,马海艳,郑顺林,向竹清,熊 湖,黄 强,方小婷.连作对马铃薯酚酸类自毒物质及根际真菌群落的影响[J].西北农业学报,2021,(3):431~438
DOI:10.7606/j.issn.1004-1389.2021.03.012
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作者单位
龚 静,马海艳,郑顺林,向竹清,熊 湖,黄 强,方小婷 (1. 四川农业大学农业部西南作物生理生态与耕作重点实验室,成都 6111302. 农业部薯类作物遗传育种重点实验室成都久森农业科技有限公司,四川新都 610500) 
基金项目:国家重点研发计划(2018YFD0200808);四川省育种攻关及配套项目(2016NYZ0051-5,2016NYZ0032);国家现代农业产业技术体系四川薯类创新团队(川财教[2019]59号)。
中文摘要:为研究连作对马铃薯根际土壤中酚酸类自毒物质及真菌群落的影响,通过收集不同连作季(1 季、3 季、5季)马铃薯根际土壤,利用高效液相色谱法测定酚酸类物质及Illumina高通量测序技术测定真菌ITS序列,结果表明:(1)连作土壤中检测出丁香酸、香豆酸、对羟基苯甲酸、阿魏酸,连作增强后,pH不断下降,对羟基苯甲酸、阿魏酸含量不断增加,丁香酸、香豆酸在连作3 季后含量最高。(2)连作 3 季及5 季根际土壤中真菌多样性及丰度均显著高于连作1 季,子囊菌门为连作土壤的优势菌门;节丛孢属为连作3 季的优势菌属,盘菌属为连作5 季的优势菌种;对连作土壤进行主成分分析,连作1 季与连作3 季真菌群落及数量相似,与连作5 季差异较大。(3)自毒物质与根际真菌群落存在相关性,丁香酸与琏格霉属,香草酸与盘菌属丰度达极显著正相关;阿魏酸与盘菌属丰度间为显著负相关。因此,连作后根际土壤中自毒物质不断积累,真菌群落结构发生改变,琏格孢霉、镰刀菌等有害真菌富集。
中文关键词:马铃薯  连作  酚酸物质  高通量测序  真菌群落
 
Effect of Continuous Cropping on Autotoxic Substances and Rhizosphere Fungi Communities in Potato
Abstract:By collecting the potato rhizosphere soil from different continuous seasons,which includ 1-season (CK), 3-season, and 5-season periods.The HPLC method was used to determinate phenolic acids, the Illumina high-throughput sequencing technology was used to determinate ITS sequences, the effect of continuous cropping on potato autotoxins and rhizosphere fungi communities was studied for providing theoretical basis to eliminate the obstacles for potato continuous cropping.The results revealed that (1) The contents of 4 phenolic acids,including syringic acid, coumaric acid, p-hydroxybenzoic acid and ferulic acid, were detected in the continuous cropping soil.The contents of phydroxybenzoic acid and ferulic acid increased continuously while the pH declined constantly. The contents of syringic acid and coumaric acid were the highest in the 3-season continuous cropping soil. (2) The diversity and abundance of fungi in the 3-season and 5-season continuous cropping rhizosphere soil were significantly higher than that of the CK. There were 6 main phyla in the fungal community while the most dominant fungi phylum was Ascomycete in the 3-season and 5-season continuous cropping soil. Arthrobotyrs was dominant in 3-season continuous cropping soil while Pepziza was dominant in that of 5-season continuous cropping. The principle component analysis of continuous cropping soil revealed that there were similar Mycoflora communities and quantities between the soil of CK and 3-season continuous cropping, which was significantly different from that of the 5-season continuous cropping. (3) There was a correlation between potato autotoxins and rhizosphere fungi communities and quantities. The abundances of syringic acid and Alternaria, vanillic acid and Peziza had significantly positive relation, the abundances of ferulic acid and Peziza had significantly negative relation. Therefore, autotoxins accumulated constantly after continuous cropping of potatoes in the rhizosphere soil, it altered the structure of fungal communities and amassed the harmful fungi such as Alternaria and Fusarium.
keywords:Solanum tuberosum L  Continuous cropping  Phenolic acid  High-throughput sequencing  Fungal communities
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