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谷子 SiMYB76基因抑制种子油脂积累的功能分析
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引用本文:刘 华 ,于芮芦,龚静云,王建军,李 东,陈明训.谷子 SiMYB76基因抑制种子油脂积累的功能分析[J].西北农业学报,2021,30(6):838~847
DOI:10.7606/j.issn.1004-1389.2021.06.006
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作者单位
刘 华 ,于芮芦,龚静云,王建军,李 东,陈明训 (西北农林科技大学 农学院陕西杨凌 712100) 
基金项目:国家自然科学基金(31971974);陕西省重点研发计划(2019NY-016);西北农林科技大学青年卓越人才培养计划(Z109022001)。
中文摘要:拟南芥AtMYB76转录因子参与调控脂肪族芥子油苷和油脂的积累过程。为深入了解谷子中的同源基因 SiMYB76在种子油脂积累过程中的调控作用,从谷子品种‘豫谷1号’中克隆获得 SiMYB76基因的全长编码域序列,对其进行烟草叶片的亚细胞定位研究,并初步分析其在种子油脂积累过程中的调控作用。结果表明,SiMYB76定位于烟草叶片细胞的细胞核中。在拟南芥 myb76-2突变体背景下,异源表达 SiMYB76基因不仅能够完全恢复突变体高含油量的表型,而且可显著调控油脂合成途径上多个关键基因的表达。由此可知,谷子SiMYB76通过调控油脂合成途径上关键基因的表达而显著抑制种子油脂积累,并在种子油脂积累过程中与拟南芥AtMYB76具有相似的生物学功能。
中文关键词:AtMYB76   SiMYB76  谷子  拟南芥  油脂积累
 
Functional Analysis of Inhibition of Seed Oil Accumulation by SiMYB76 Gene in Foxtail Millet
Abstract:AtMYB76 transcription factor is involved in regulating the accumulation of aliphatic glucosinolates and oil in Arabidopsis thaliana. In order to further understand the role of the foxtail millet homologous gene SiMYB76 in the regulation of seed oil accumulation, we cloned the full length coding domain sequence of SiMYB76 gene from the S.italica cultivar ‘Yugu1’to investigate its subcellular localization in tobacco leaf cells, and we also preliminarily analyzed its regulatory role in seed oil accumulation in A.thaliana. The results showed that SiMYB76 was located in the nucleus of tobacco leaf cells.Under the background of A.thaliana myb76-2 mutant, the hetero expression of SiMYB76 gene not only completely restored the higher oil content phenotype of the mutant, but also significantly regulated the expression of several key genes in the oil biosynthetic pathway. Therefore,SiMYB76 significantly inhibits seed oil accumulation by regulating the expression of key genes in the oil biosynthetic pathway, and exhibits a similar biological function with AtMYB76 during seed oil accumulation.
keywords:AtMYB76   SiMYB76  Setaria italica  Arabidopsis thaliana  Oil accumulation
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