| 西瓜 PIP5K基因家族鉴定及其在雄性不育花蕾中的表达分析 |
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| 引用本文：张高原，丁 谦，魏兵强.西瓜 PIP5K基因家族鉴定及其在雄性不育花蕾中的表达分析[J].西北农业学报,2021,30(6):883~893 |
| DOI:10.7606/j.issn.1004-1389.2021.06.010 |
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| 基金项目:甘肃农业大学公开招聘博士科研启动基金（2017RCZX-30）。 |
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| 中文摘要:为了挖掘西瓜磷脂酰肌醇4-磷酸5-激酶（PIP5K）基因的功能，利用生物信息学方法对西瓜PIP5K家族成员进行鉴定和特征分析，并对其在多个组织的表达模式进行分析研究。结果显示：西瓜基因组中含有8个PIP5K家族成员，分布在6条染色体上；多数基因定位在细胞质或细胞膜上；共线性分析发现4个 ClaPIP5K基因与5个 AtPIP5K基因存在共线关系，并且 ClaPIP5K基因间存在片段复制事件；进化树分析发现 ClaPIP5K基因和 AtPIP5K基因被分成4组，并且同一组内的基因具有相似的保守基序和基因结构；启动子分析发现 ClaPIP5K基因中含有激素和胁迫响应相关顺式作用元件；表达分析显示 ClaPIP5K基因在西瓜生长点、果柄、果肉、叶片、下胚轴、根以及花蕾等组织中表现出不同的表达水平。 |
| 中文关键词:西瓜 PIP5K 基因家族 雄性不育 表达模式 |
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| Identification of PIP5K Gene Family in Watermelon (Citrullus lanatus) and Its Expression Analysis in Male Sterile Floral Buds |
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| Abstract:In order to explore the function of phosphatidylinositol 4-phosphate 5-kinase (PIP5K)genes in watermelon,the watermelon PIP5K gene family was identified and their characteristics and expression patterns were analyzed by using bioinformatics method in this study. The results showed that there were 8 PIP5K members in watermelon,which were distributed on six chromosomes. Most genes were located in cytoplasm or cell membrane. Syntenic analysis showed that there were synteny relationships between 4 ClaPIP5K and 5 AtPIP5K genes,and one ClaPIP5K gene pair was identified as segmental duplication.The phylogenetic tree analysis showed that ClaPIP5K and AtPIP5K genes were divided into 4 groups,and the genes had similar conserved motifs and gene structure in the same group. The promoter analysis showed that the ClaPIP5K genes contained cis-acting elements related to hormone and stress response. The expression pattern analysis showed that ClaPIP5K genes displayed different expression levels in the apical point,carpopodium,fruit flesh,leaf,hypocotyl,root and floral bud of watermelon. |
| keywords:Watermelon PIP5K Gene family Male sterile Expression pattern |
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