Abstract:In order to understand the effect of combined application of Bacillus Floridans gz4-1 and straw on the growth and development of tartary buckwheat seedlings, taking 'Diqing' tartary buckwheat as material in 2019,using normal fertilization as control (CK), two factors combined treatment of straw [0 g·kg-1 soil (J0), 4 g·kg-1 soil (J1)] and bacterial liquid concentration [0 (P0),10 (P1),106 (P2),108 (P3),1010 (P4)cfu·mL-1] were set in this paper.A pot experiment was conducted to investigate the effects of combined application of Bacillus Floridans gz4-1 and straw on root and shoot morphology and physiological characteristics of tartary buckwheat seedlings.The results showed that:(1) The combined application of Bacillus Floridans gz4-1 and straw promoted the root surface area and root shoot ratio of tartaric buckwheat root fresh mass, dry mass, taproot length and root shoot ratio.The taproot length of seedlings was the highest under J0P2 treatment, which significantly increased by 12.1% compared with CK.The root surface area and root shoot ratio were both the highest under J1P1 treatment, which significantly increased by 115.6% and 103.3% compared with CK.(2) The combined application of Bacillus Floridans gz4-1 and straw promoted the stem diameter, plant height, fresh mass, dry mass and node number of tartary buckwheat seedlings, the plant height and node number were the highest under the treatment of J1P3, which were 43.6% and 33.3% higher than CK.(3) With the increase of bacterial solution concentration, root activity and root P content of tartary buckwheat seedlings increased firstly and then decreased, and reached the highest value under P2 concentration.Under low phosphorus cultivation (J0) condition, root acid phosphatase activity decreased, and under condition of straw returning to field (J1), root acid phosphatase activity reached the peak value under P2 concentration.That Bacillus Floridans gz4-1 and straw could improve root activity, root physiological function by changing the buckwheat seedling morphology and root architecture,finally, its effect on the buckwheat seedling growth was improved.Comprehensively, under treatment of 106 cfu·mL-1 (P2) bacteria liquid concentrations, combined application of straw had the greatest growth-promoting effect on tartary buckwheat seedlings. |