| 遏蓝菜硒蛋白的纯化、结构表征及抗氧化活性研究 |
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| 引用本文：向安妮，许 爽，鞠红梅，赵诗宇，岳田利，袁亚宏.遏蓝菜硒蛋白的纯化、结构表征及抗氧化活性研究[J].西北农业学报,2022,(3):299~309 |
| DOI:10.7606/j.issn.1004-1389.2022.03.005 |
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| 基金项目:国家“十三五”重点研发计划（2019YFC1606702-02）。 |
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| 中文摘要:为探究遏蓝菜硒蛋白的结构特征及抗氧化活性，采用超声辅助酶提法和DEAE-Sepharose FF阴离子交换柱层析法，从遏蓝菜中提取、分离和纯化出两种硒蛋白组分（PSP-2和PSP-3），运用光谱法和扫描电镜等方法对其结构进行表征，并检测其体外抗氧化活性。结果表明:PSP-2和PSP-3各含有15种氨基酸，包括6种必需氨基酸，两种蛋白组分氨基酸组成合理，具有良好的营养价值；PSP-2和PSP-3具有典型的蛋白光谱特征，均存在Se=O键、O-Se-O键、氢键和二硫键，结构不稳定并呈现出聚集体及碎片的外观形貌；抗氧化活性检测表明，PSP-2和PSP-3具有较强的自由基清除能力和总还原力。综上所述，本研究证实遏蓝菜中存在具有优良抗氧化活性的天然硒蛋白，为进一步研究遏蓝菜硒蛋白的构效关系和开发具有抗氧化功效的产品提供了依据，可为遏蓝菜资源的综合利用提供参考。 |
| 中文关键词:遏蓝菜 硒蛋白 分离纯化 结构表征 抗氧化分析 |
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| Research on Purification,Structure Characterization and Antioxidant Activity of Selenoproteins in Thlaspi arvense L. |
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| Abstract:To explore the structure characteristics and antioxidant activity of selenoproteins in Thlaspi arvense L.two selenoprotein components of PSP-2 and PSP-3,were extracted and purified from Thlaspi arvense L.by ultrasonic-assisted enzyme extraction and DEAE Sepharose FF anion exchange column chromatography.The structure was characterized and the antioxidant activity in vitro was evaluated by analysis methods such as spectroscopy and scanning electron microscopy.The results showed that PSP-2 and PSP-3 contained 15 amino acids,in which 6 were essential amino acids.The amino acids of the two protein components were reasonably composed and had good nutritional values.PSP-2 and PSP-3 had typical protein spectral characteristics,both of which had Se=O bond, O-Se-O bond,disulfide bond and H-bond,and the unstable structures showed the appearance of aggregates and fragments.This study on antioxidant activity showed that PSP-2 and PSP-3 had strong free radical scavenging ability and total reducing power.In summary,the selenoproteins with excellent antioxidant activity existed in Thlaspi arvense L.Furthermore,this experiment provided a basis for further study on the structure-activity relationship of selenoproteins in Thlaspi arvense L.and developing products with antioxidant effect,and also provided a reference for the comprehensive utilization of Thlaspi arvense L. |
| keywords:Thlaspi arvense L. Seleno protein Extraction and purification Structure characterization Antioxidant activity analysis |
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