| 基于SSR标记辣椒品种DNA指纹图谱的构建 |
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| 引用本文：冯鹏龙，韩 睿，王亚艺，邵登魁，李全辉 ，钟启文.基于SSR标记辣椒品种DNA指纹图谱的构建[J].西北农业学报,2022,(3):320~327 |
| DOI:10.7606/j.issn.1004-1389.2022.03.007 |
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| 基金项目:青海省农林科学院创新基金（2019-NKY-03）；国家自然科学基金（31860556）；青海省科技计划（2021-ZJ-763）；青海省科技厅重点实验室项目（2020-ZJ-Y02）。 |
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| 中文摘要:基于辣椒全基因组开发的SSR标记，对青海地区主栽的26份主栽辣椒品种进行遗传多样性分析并构建分子指纹图谱。结果从117对SSR标记中筛选出6对扩增稳定，多态性较好的引物，6对标记共扩增到36个基因位点，多态性比例为97%；26份青海地区主栽辣椒品种之间遗传相似系数为0.42～1.00，在GS 0.5处可将26份辣椒材料聚为4类。优选pepperSSR01、pepperSSR04、pepperSSR06、pepperSSR08这4对引物组合便可准确快速地区分供试辣椒材料。 |
| 中文关键词:辣椒 SSR标记 遗传多样性 指纹图谱 |
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| Construction of DNA Fingerprints of Pepper(Capsicum annuum L.) Varieties Based on SSR Markers |
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| Abstract:Based on SSR markers developed for the whole pepper genome,the genetic diversity of 26 major pepper cultivars grown in Qinghai were analyzed in current study,and their molecular fingerprints were constructed.The results showed that 6 pairs of primers with stable amplification and good polymorphism were selected from 117 pairs of SSR markers,and a total of 36 gene loci were amplified from 6 pairs of markers,with a polymorphism ratio of 97%.The genetic similarity coefficient among 26 pepper varieties was 0.37-1.00,and the 26 pepper materials could be grouped into 4 types at GS 0.5. Preferably,the four pairs of primers (pepperSSR01,pepperSSR04,pepperSSR06 and pepperSSR08) could distinguish the tested pepper materials accurately and quickly. |
| keywords:Pepper SSR marker Genetic diversity Fingerprint |
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