| 麦田土壤解磷细菌的筛选及其解磷能力研究 |
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| 引用本文：孙亚钦，叶盛嘉，范国安，张 影，刘 星，吴大付，王 菲.麦田土壤解磷细菌的筛选及其解磷能力研究[J].西北农业学报,2022,(3):379~387 |
| DOI:10.7606/j.issn.1004-1389.2022.03.014 |
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| 基金项目:国家自然科学基金（31872184）；河南科技学院大学生创新训练计划（2021CX45）；河南科技学院高层次人才科研项目（2016031）。 |
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| 中文摘要:选用以植酸钙为磷源的培养基，对麦田土壤中的解磷细菌进行分离筛选，利用16S rRNA基因测序技术鉴定解磷细菌种类并构建系统进化树，根据培养液的实际pH测定其磷酸酶活性，利用溶磷圈法和钼锑抗比色法对其解磷能力进行测定。从麦田土壤中共筛选鉴定出5株解磷菌株，分别隶属于Bacillus、Arthrobacter和Pseudarthrobacter；5株解磷菌株对应的培养液pH显著下降，实际磷酸酶活性为5.18~6.39 μg/(mL·min)，从植酸钙中活化出的速效磷含量为2.49~3.55 mg/L，活化率为19.28%~43.54%，其对应培养液中的磷酸酶活性与pH以及速效磷含量之间分别呈现极显著的负相关关系和正相关关系。结果表明解磷能力最强的是菌株FLP36，属于Bacillus。 |
| 中文关键词:解磷细菌 溶磷圈 有机磷 磷酸酶活性 解磷能力 |
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| Isolation and Phosphate Solubilizing Capability of Phosphate-Solubilizing Bacteria in a Wheat Field |
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| Abstract:In the present study, phytin, a kind of organic P, was chosen as sole P source in the medium to isolate phosphate-solubilizing bacteria in the soil collected form a wheat field. Identification of phosphate-solubilizing bacteria and construction of phylogenetic tree was subjected to 16S rRNA gene sequencing technology. The phosphatase activity of the isolated phosphate-solubilizing bacterial strains was determined according to the actual pH of the culture medium. The phosphate-solubilizing ability was measured by the combination of the solubilization halo method and molybdenum blue method. Five phosphate-solubilizing bacterial strains were identified and affiliated to the genus of Bacillus, Arthrobacter and Pseudarthrobacter, respectively. Compared with the initial pH, the pH of the culture medium corresponding to the five phosphate-solubilizing strains was significantly decreased and the actual phosphatase activity ranged from 5.18 to 6.39 μg/(mL·min).The concentration of soluble P mobilized from phytin ranged from 2.49 to 3.55 mg/L and the mobilization ratio ranged from 19.28% to 43.54%. The phosphatase activity in the corresponding liquid medium was significantly and negatively correlated with pH, while was significantly and positively correlated with soluble P concentration. The strain with most powerful capability to mobilize phytin belonged to Bacillus. These results provide a theoretical basis for studying on the mobilization of soil organic P by phosphate-solubilizing bacteria and useful reference for development and application of microbial fertilizers. |
| keywords:Phosphate-solubilizing bacteria Phosphate-solubilization halo Organic P Phosphatase activity Phosphate solubilizing capability |
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