| 养殖池塘底泥中恩诺沙星对氮转化的影响 |
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| 引用本文：刘立早，张玉平，张玲玲.养殖池塘底泥中恩诺沙星对氮转化的影响[J].西北农业学报,2023,(5):753~761 |
| DOI:10.7606/j.issn.1004-1389.2023.05.011 |
| 摘要点击次数: 51 |
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| 基金项目:上海市自然科学基金项目（20ZR1450800）;国家自然科学基金（51709172，31900097）。 |
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| 中文摘要:为了研究水产养殖池塘底泥中抗生素残留对氮赋存形态及氮转化功能微生物的影响。选取养殖环境中常见的恩诺沙星（ENR）为目标污染物，设置0、1.0、2.0、5.0 mg/kg 4 个梯度开展模拟试验。结果显示：底泥中NH+4-N和NO-3-N含量随试验开展呈下降趋势，添加ENR后，底泥中NO-2-N含量增加 7.55%~15.30%；底泥氮转化相关基因中，anammox相对丰度最高，占比60.0%~78.4%。厌氧氨氧化是底泥中氮转化的主要途径，底泥中NH+4-N含量与anammox丰度呈极度负相关；底泥中低含量ENR（1.0 mg/kg）会促进nxrA基因的生长或繁殖，但含量超过2.0 mg/kg时对反硝化基因nirK有明显抑制作用。 |
| 中文关键词:养殖池塘 底泥 恩诺沙星 氮转化 功能基因 |
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| Effect of Enrofloxacin on Nitrogen Transformation in Sediment of Aquacultural Ponds |
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| Abstract:To explore the effect of antibiotics on nitrogen forms and related microorganism in sediment of aquacultural ponds,enrofloxacin(ENR) was selected as object，four concentration gradients (0,1.0,2.0 and 5.0 mg/kg) were set up in this study. The concentrations of NH+4-N and NO-3-N in the sediment decreased with the processing of the experiment. After addition of ENR,the NO-2-N concentrations in sediment increased by 7.55%-15.30% .The relative abundance of anammox in the sediment was the highest in the N transformation genes,accounting for 60.0%-78.4%. Anaerobic ammonia oxidation played dominant role in the sediment. The abundance of anammox had significantly negative correlation with NH+4-N concentration. Low concentration of ENR(1.0 mg/kg) in the sediment promoted the growth of nxrA gene. The growth of nirK gene was inhibited when the concentration of ENR was more than 2.0 mg/kg. |
| keywords:Aquaculture ponds Sediment Enrofloxacin Nitrogen transformation Functional genes |
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