Abstract:In order to illustrate the effect of microbial agents on the bacterial community structure of potato rhizosphere soil from the perspective of microbial ecology,the high-throughput sequencing technology was used to sequence and analyze the sequence of the bacterial 16S rRNA V3+V4 region,CK(no fertilizer) and F(single application of chemical fertilizers) were used as controls,the diversity analysis of rhizosphere soil bacterial communities was studied under treatments of T1 (fertilizer + granular agent 75 kg·hm-2),T2 (chemical fertilizer + granular agents 150 kg·hm-2),T3 (chemical fertilizer + granular agents 225 kg·hm-2) and T4 (chemical fertilizer + granular agents 300 kg·hm-2).The results showed that T2 could significantly increase the number of OTU,Chao1 index and ACE index compared with F.Compared with CK at the F level,T4 Acidobacteria decreased by 33.24%,29.33%,respectively.Gemmatimonadetes decreased by 25.16%,21.01%,respectively.Compared with CK at the class level,the relative abundances of T4 Gammaproteobacteria and Bacteroidia increased by 32.04% and 59.73%,respectively,and the relative abundances of T3 and T4 Actinobacteria increased by 129.11% and 169.68%,respectively,the relative abundances of T4 Gemmatimonadetes and Blastocatellia_Subgroup_4 decreased by 26.91% and 38.1%,respectively; compared with F,the relative abundances of T3 and T4 Acidimicrobiia decreased by 21.38% and 24.04%,respectively,while the relative abundances of Actinobacteria increased by 59.48% and 87.72%,respectively.The relative abundance of T2,T3 and T4 Bacteroidia increased by 74.19%, 73.52%,124.49%,respectively.This study indicated that the application of microbial agents significantly increases the abundance of soil bacteria,improves the microbial community structure,and increases the abundance of beneficial bacteria,and then improves the soil microenvironment of potato. |