| 云杉叶枯病新病原的鉴定及其生物学特性测定 |
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| 引用本文：李柘柘，崔凌霄，魏立娟，金梦军，马 婷，杨成德.云杉叶枯病新病原的鉴定及其生物学特性测定[J].西北农业学报,2023,(5):791~798 |
| DOI:10.7606/j.issn.1004-1389.2023.05.015 |
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| 基金项目:甘肃省林草局项目（GFG2019-366-3）。 |
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| 中文摘要:在甘肃省天水市林区采集到一种症状与已报道略有差别的云杉叶枯病，对病原菌进行分离与鉴定，并测定其致病性和生物学特性。结果表明，在云杉上引起叶枯病症状的分离物SJ1菌丝呈白色、红橙色、深黄棕色，后期菌落表面出现红黑色液滴，分生孢子黑色，扁圆形或近球形，表面有褶皱。 ITS、 TUB2和 RPB2基因序列分析表明其与黑附球菌（Epicoccum nigrum）同源性最高，且在系统发育树上聚在一起，因此将其鉴定为黑附球菌（E.nigrum）。生物学特性测定结果表明，最适菌丝生长的培养基为麦芽浸粉琼脂培养基，温度为25 ℃，pH为7，光照对菌丝生长影响不大，最适碳、氮源分别为淀粉和蛋白胨，氮源为尿素时不生长。 |
| 中文关键词:云杉叶枯病 黑附球菌 鉴定 生物学特性 |
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| Identification of A Novel Pathogen of Needle Blight of Picea asperata and Its Biological Characteristics |
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| Abstract:In the forest region of Tianshui in Gansu province, a Picea asperata needle blight with symptom slightly different from those previously reported ones we collected.The pathogenic fungi were isolated, identified, and their pathogenicity and biological characteristics were assessed.The results revealed that the mycelial of the isolate SJ1 inducing needle blight symptoms on P.asperata was white, reddish-orange and dark yellowish-brown in color, had red black droplets on the anaphase colony’s surface.Conidia had wrinkles on the surface and were dark, oblate or nearly spherical.The ITS, TUB2 and RPB2 genes had the most identity with Epicoccum nigrum and clustered together on the phylogenetic tree, indicating that the pathogen SJ1 was E.nigrum.According to the biological characteristics, malt extract agar was the best medium for E.nigrum.The optimum temperature for mycelial growth was 25 ℃, the pH was 7, the light conditions had minimal effect on mycelial growth, the carbon and nitrogen sources were starch and peptone, the mycelial did not grow when the nitrogen source was urea. |
| keywords:P.asperata needle blight E.nigrum Identification Biological characteristics |
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