| 陕西省西洋参根结线虫病的发生和病原鉴定 |
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| 引用本文：耿英杰，陈德经，商文静.陕西省西洋参根结线虫病的发生和病原鉴定[J].西北农业学报,2023,(5):799~806 |
| DOI:10.7606/j.issn.1004-1389.2023.05.016 |
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| 基金项目:陕西省区域创新能力引导计划（2020QFY12-06）；秦巴生物资源与生态环境省部共建国家重点实验室开放基金（SLGPT2019KF04-03）；西北农林科技大学试验示范站（基地）科技成果推广项目（TGZX2019-32） |
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| 中文摘要:为明确陕西省西洋参根结线虫病病原，采用常规形态鉴定结合分子生物学方法，对在陕西省留坝县严重发生的西洋参根结线虫病进行病原鉴定。结果表明：二龄幼虫蠕虫形，口针基部球小而圆，尾部透明区明显，尾尖钝圆；雌虫虫体梨形或近椭圆形，口针杆部后部稍宽，口针基部球近圆形较小有缢缩。雌虫会阴花纹呈近圆形，线纹平滑呈波浪状，尾端区有刻点，背弓较为低平。结合特异性PCR扩增及根结线虫属rDNA-ITS序列系统发育树构建结果，该病原鉴定为北方根结线虫Meloidogyne hapla。 |
| 中文关键词:西洋参 北方根结线虫 病原鉴定 |
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| Pathogen Identification of Panax quinquefolium Root-knot Nematode Disease in Shaanxi of China |
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| Abstract:In order to clarify the pathogen species of Panax quinquefolium root-knot nematode disease in Shaanxi ,the root samples with obvious root-knots of Panax quinquefolium were collected for isolating root-knot nematodes from P.quinquefolium planting area in Liuba County,Shaanxi Province.The pathogenic nematodes were identified by observing morphological characteristics of females,second-stage juveniles and perineal pattern,sequence alignments,phylogenetic analysis and specific PCR amplification.The results showed that the pathogen species of P.quinquefolium root-knot nematode disease in Shaanxi was M.hapla. |
| keywords:American ginseng Meloidogyne hapla Pathogen identification |
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