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利用酵母双杂交系统筛选玉米ZmPRR73的互作蛋白
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引用本文:王盼盼,王雷立,张严玲,刘青青,董柯清,李安然,王翠玲.利用酵母双杂交系统筛选玉米ZmPRR73的互作蛋白[J].西北农业学报,2024,(3):416~525
DOI:10.7606/j.issn.1004-1389.2024.03.005
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作者单位
王盼盼,王雷立,张严玲,刘青青,董柯清,李安然,王翠玲 (河南科技大学 农学院,河南洛阳 471023) 
基金项目:国家自然科学基金河南省联合基金(U2004153);河南省重点研发与推广专项(科技攻关)项目(202102110010);国家自然科学基金青年基金(31901548,31901561)。
中文摘要:为了阐明ZmPRR73基因的生物学功能,构建诱饵表达载体pGBKT7-ZmPRR73,利用酵母双杂交技术,从长日照光照环境诱导的热带玉米自交系的cDNA文库中筛选与ZmPRR73互作的蛋白。结果显示:构建的诱饵载体pGBKT7-ZmPRR73对酵母菌株无毒性,且对报告基因无自激活活性,共鉴定出12个与ZmPRR73互作的候选蛋白。生物信息学分析表明,这些候选互作蛋白的功能涉及植物的转录调控、离子跨膜转运的调节、信号转导、电子传递链等多个方面,推测ZmPRR73蛋白与以上蛋白互作参与多个信号转导和代谢途径,研究结果补充和完善了ZmPRR73蛋白参与的调控途径,为进一步研究生物钟核心元件ZmPRR73的分子功能提供了新的分子证据。
中文关键词:玉米  酵母双杂交  ZmPRR73  生物钟  互作蛋白
 
Screening of Proteins Interacting with ZmPRR73 in Maize Using Yeast Two-hybrid System
Abstract:To elucidate its biological function,the bait vector PGBKT7-ZMPRR73 was constructed,and the proteins interacting with ZmPRR73 was screened from the yeast cDNA library of a tropical maize inbred line induced by long-day photoperiod treatment,using yeast two-hybrid technique.The results showed that the bait vector pGBKT7-ZmPRR73 exhibited no toxicity to yeast strains and did not activate the reporter gene on its own.A total of 12 candidate proteins that interacted with ZmPRR73 were identified.Bioinformatics analysis showed that these candidate interacting proteins were involved in various pathways,such as plant transcription regulation,ion transmembrane transport regulation,signal transduction and electron transport chain.This suggests that ZmPRR73 may participate in multiple signal transduction and metabolic pathways by interacting with the identified interaction proteins.The results enhance our understanding of the signal transduction and the regulatory pathways associated with ZmPRR73 and provide molecular evidence for further investigations into its molecular function and regulatory mechanism as a core component of circadian clock in maize.
keywords:Maize  Yeast two-hybrid system  ZmPRR73  Circadian  Interacting protein
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