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獭兔胃肠道乳酸菌的分离与鉴定
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引用本文:张菲菲,薛海涛,吴 婕,任战军.獭兔胃肠道乳酸菌的分离与鉴定[J].西北农业学报,2024,(7):1208~1214
DOI:10.7606/j.issn.1004-1389.2024.07.003
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作者单位
张菲菲,薛海涛,吴 婕,任战军 (1.乌鲁木齐海关乌鲁木齐 8300002.西北农林科技大学 动物科技学院陕西杨凌 712100) 
基金项目:陕西省农业科技创新驱动项目[NYKJ-2021-YL(XN)28]。
中文摘要:为探索獭兔胃肠道中固有的乳酸菌群,丰富乳酸菌应用,选择中性或偏酸性MRS培养基分离并培养健康獭兔胃肠道中的乳酸菌、16S rDNA测序对所获菌株进行种属鉴定、基因组DNA荧光定量PCR检测其在40、80日龄的獭兔胃肠道中的相对含量。结果从獭兔胃、空肠、盲肠、结肠、直肠中均分离出1株粪肠球菌;胃、十二指肠、空肠、回肠、盲肠中均分离出1株短乳杆菌;胃、十二指肠、回肠、盲肠、结肠、直肠中均分离出1株植物乳杆菌;十二指肠、空肠、盲肠、结肠中均分离出1株空肠肠球菌。所获菌株在獭兔胃肠道中普遍分布,并且其相对水平随獭兔日龄的增长而升高,这些菌类有待成为獭兔内源乳酸菌饲料添加剂的备用选择。
中文关键词:乳酸菌  獭兔  16S rDNA
 
Isolation and Identification of Lactic Acid Bacteria in Gastrointestinal Tract of Rex Rabbits
Abstract:In order to explore the inherent lactic acid bacteria in the gastrointestinal tract of rex rabbits and enrich the application of lactic acid bacteria,the neutral or acidic MRS medium was used to isolate and culture the lactic acid bacteria from the gastrointestinal tract of healthy rex rabbits.The species of the strains were identified by 16SrDNA sequencing,and the relative content of the strains in the gastrointestinal tract of 40-and 80-day-old rex rabbits was detected by genomic DNA fluorescence quantitative PCR.The results were as follows: Enterococcus faecalis strains were isolated from the stomach,jejunum,cecum,colon and rectum; Lactobacillus brevis strains were isolated from stomach,duodenum,jejunum,ileum and cecum; Lactobacillus plantarum strains were isolated from stomach,duodenum,ileum,cecum,colon and rectum respectively; Enterococcus hirae strains were isolated from duodenum,jejunum,cecum and colon.The strains obtained in this experiment are widely distributed in the gastrointestinal tract of rex rabbits,and their relative level increases with the age of rex rabbits,which suggests that these bacteria can be an alternative choice of endogenous lactic acid bacteria feed additives for rex rabbits.
keywords:Lactic acid bacteria  Rex  16S rDNA
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