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茄子转录因子 SmeWRKY53对盐胁迫的响应
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引用本文:周亚茹,肖 凯,张爱冬,吴雪霞.茄子转录因子 SmeWRKY53对盐胁迫的响应[J].西北农业学报,2024,(7):1290~1307
DOI:10.7606/j.issn.1004-1389.2024.07.011
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作者单位
周亚茹,肖 凯,张爱冬,吴雪霞 (1.上海海洋大学 水产与生命学院上海 2013062.上海市农业科学院 园艺研究所上海 201403) 
基金项目:国家大宗蔬菜产业技术体系项目(CARS-25)。
中文摘要:WRKY转录因子在各种生理过程和逆境反应中发挥重要作用,为了探究茄子转录因子 SmeWRKY53抗逆性,对 SmeWRKY53的启动子元件进行预测分析,运用实时荧光定量PCR(RT-qPCR)检测 SmeWRKY53在不同非生物胁迫中表达量的变化,通过遗传转化在茄子中过表达 SmeWRKY53,并对过表达茄子苗期耐盐性进行鉴定。启动子元件预测分析结果显示,该基因启动子序列不仅有启动子基本转录元件,还含有TC-rich repeats、CGTCA-motif、TGACG-motif等与胁迫相关的抗逆元件。实时荧光定量(qRT-PCR)结果显示 SmeWRKY53能够响应高温、低温、干旱以及盐胁迫处理,且在盐胁迫条件下可以被显著诱导。在茄子中过表达 SmeWRKY53可以提高植株的耐盐能力。生理指标检测表明,盐胁迫条件下,过表达植株体内丙二醛(MDA)含量积累显著低于对照。同时过表达植株细胞内脯氨酸、可溶性蛋白的含量和抗氧化酶\[过氧化氢酶(CAT)和过氧化物酶活(POD)\]的活性显著高于对照植株。对盐胁迫相关基因( SmeSOS1 SmeCIPK3 SmeNHX1)的表达检测发现,盐胁迫处理显著诱导了相关基因( SmeSOS1 SmeCIPK3 SmeNHX1)的表达,且过表达植株中的表达显著高于对照。这些结果表明茄子 SmeWRKY53能正调控提高茄子对盐胁迫的耐受性,对后续研究 SmeWRKY53基因在茄子耐盐胁迫中的分子机制提供了理论依据。
中文关键词:茄子  转录因子   SmeWRKY53  非生物胁迫  耐盐性
 
Response of Eggplant Transcription Factor SmeWRKY53 to Salt Stress
Abstract:WRKY transcription factor plays an important role in various physiological processes and stress responses.To explore the stress resistance of eggplant transcription factor SmeWRKY53,the promoter elements of SmeWRKY53 were predicted and analyzed.Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression changes of SmeWRKY53 under different abiotic stresses. SmeWRKY53 was overexpressed in eggplant through genetic transformation,and the salt tolerance of the overexpressed eggplant at seedling stage was identified.The results of promoter element prediction analysis showed that the promoter sequence of this gene not only contains basic transcriptional elements of promoters,but also contains stress-related elements such as TC-rich repeats,CGTCA-motif and TGACG-motif.Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that SmeWRKY53 could respond to high temperature,low temperature,drought and salt stress in eggplant,and could be significantly induced under salt stress.Physiological tests showed that the accumulation of malondialdehyde (MDA) in overexpressed plants was significantly lower than that in control under salt stress.Meanwhile,overexpression of SmeWRKY53 increased the contents of proline and soluble protein and the activities of antioxidant enzymes (Catalase (CAT) and Peroxidase activity (POD)) to improve the salt tolerance of plants.The expression of salt stress-related genes (SmeSOS1,SmeCIPK3 and SmeNHX1) was detected,and the expression levels of salt stress-related genes (SmeSOS1,SmeCIPK3 and SmeNHX1) in overexpressed plants were significantly increased after salt stress treatment compared with the wild type.These results suggest that eggplant SmeWRKY53,as a positive regulator,can improve the tolerance of eggplant to salt stress.
keywords:Eggplant  Transcription factor   SmeWRKY53  Abiotic stress  Salt tolerance
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