| 基于蛋白质组学研究西瓜接穗对嫁接苗根系生长的影响 |
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| 引用本文：赵加欣，谢露露，温正阳，孙靖博，张 锋，张梦夏，董春娟.基于蛋白质组学研究西瓜接穗对嫁接苗根系生长的影响[J].西北农业学报,2024,(8):1480~1482 |
| DOI:10.7606/j.issn.1004-1389.2024.08.008 |
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| 基金项目:国家重点研发计划（2019YFD1001901）；中国农业科学院科技创新工程项目（CAAS ASTIP IVFCAAS，CAAS ZDRW 202302）；国家现代农业产业技术体系建设专项（CARS 25）。 |
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| 中文摘要:为研究嫁接苗中西瓜接穗对南瓜砧木根系生长的影响，以西瓜品种‘早佳8424’、南瓜品种‘伟砧1号’为试验材料，设置两个嫁接组合，西瓜/南瓜（W/P）和南瓜/南瓜（P/P），采用贴接法嫁接，并以不嫁接的南瓜自根苗（P）为对照，采用iTRAQ技术对W/P、P/P嫁接苗及P的根系蛋白质组进行全面分析和比较。结果表明W/P和P/P嫁接苗的砧穗愈合良好，但在愈合期结束后，W/P嫁接苗的地上部和根系生长最为缓慢。iTRAQ结果表明相较于P，W/P根系中共鉴定到194个差异丰度蛋白，其中上调蛋白135个、下调蛋白59个；相较于P/P，W/P根系中共鉴定到54个差异丰度蛋白，其中上调20个、下调蛋白34个。这些差异丰度蛋白主要富集在羧酸代谢、碳水化合物代谢、逆境胁迫响应等过程。W/P嫁接苗的根系中参与蔗糖代谢、糖酵解和三羧酸循环的酶，如蔗糖合成酶、果糖激酶、丙酮酸激酶和磷酸烯醇式丙酮酸羧化酶等丰度显著低于P/P和P。W/P嫁接苗的根系中胁迫相关蛋白的丰度显著升高，包括多个类乳胶蛋白、苯丙氨酸解氨酶、水孔蛋白等。说明W/P嫁接苗中西瓜接穗可抑制砧木根系中的碳水化合物代谢、促进根系的逆境胁迫响应。 |
| 中文关键词:西瓜 南瓜 嫁接 蛋白质组学 iTRAQ 根系 |
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| Effects of Watermelon Scions on Root Growth in Grafted Seedlings by Proteomic Analysis |
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| Abstract:In order to explore the effects of watermelon scions on root growth of pumpkin rootstocks,the watermelon cultivar ‘Zaojia 8424’and pumpkin cultivar ‘Weizhen No. 1’were used as materials.Pumpkin seedlings without grafting (P) were used as control,and two grafting combinations (watermelon/pumpkin (W/P) and pumpkin/pumpkin (P/P)) were set,using the one cotyledon splice grafting method. iTRAQ method was used to compare the proteome of roots of W/P,P/P and P. The results showed that scions and rootstocks of W/P and P/P grafted seedlings could be united well. However, after the healing period,both shoot and root growth of the W/P grafted seedlings were the slowest. This suggests that although the scions and rootstocks of W/P and P/P grafted seedlings in this study could unite effectively,the W/P grafted seedlings exhibited slower shoot and root growth after the healing period. iTRAQ proteomic analysis identified 194 differential abundance proteins in W/P vs P,including 135 up related proteins and 39 down regulated proteins. Additionally, 54 differential abundance proteins were identified in W/P vs P/P,consisting of 20 up related proteins and 34 down regulated proteins. These differential abundance proteins were mainly enriched in carboxylic acid metabolic processes,carbohydrate metabolic processes,stress response processes,and so on. The abundance of enzymes involved in sucrose metabolism,glycolysis,and TCA cycle in the roots of W/P grafted seedlings,such as sucrose synthase,fructose kinase,pyruvate kinase,and phosphoenolpyruvate carboxylase,was significantly lower than that of P/P and P. The abundance of stress related proteins in the roots of W/P grafted seedlings was significantly increased,including major latex protein (MLP) like proteins,phenylalanine ammonia lyases and aquaporins. These results indicate that watermelon scion in W/P grafted seedlings can repress carbohydrate metabolism,but promote stress responses in the roots of pumpkin rootstock. |
| keywords:Watermelon Pumpkin Grafting Proteomic iTRAQ Root |
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