| 犬源细小病毒兰州株的分离鉴定与VP2基因的遗传进化分析 |
| 点此下载全文 |
| 引用本文：孔繁丽，卢曾军，陈慧丽，李 坤，刘 果，张小丽.犬源细小病毒兰州株的分离鉴定与VP2基因的遗传进化分析[J].西北农业学报,2024,(11):2011~2019 |
| DOI: |
| 摘要点击次数: 196 |
| 全文下载次数: 141 |
|
| 基金项目:甘肃农业大学公招博士科研启动基金（GAU-KYQD-2021-221010）；甘肃省高等学校创新基金（2022A-049）；甘肃省科协创新驱动助力工程项目（GXH20230817-4）。 |
|
| 中文摘要:为了解近年来甘肃省兰州市犬细小病毒（Canine parvovirus，CPV）的基因型分布情况及遗传进化方向，于兰州市宠物医院采集8份细小病毒阳性（试纸条检测）的犬粪便样品，使用F81细胞分离病毒；利用PCR、间接免疫荧光试验、血凝试验鉴定所分离的病毒；随后分析病毒基因型和 VP2基因的遗传进化关系。结果显示，共分离得到7株细小病毒，1株为New CPV-2a型CPV，5株为CPV-2c型CPV，1株为猫细小病毒（Feline parvovirus，FPV）。VP2氨基酸序列同源性及进化分析结果显示，分离到的CPV-2c型毒株VP2同源性为100%，与北京、江苏等地分离株的亲缘关系较近；New CPV-2a型毒株与吉林、西安等地的分离株亲缘关系较近；犬源猫细小病毒（LZ05）与已报道的FPV毒株相比，存在91S→A、322V→T独特的突变，且与2020年北京一株犬源猫细小病毒分离株亲缘关系最近。 |
| 中文关键词:犬细小病毒 分离鉴定 VP2 遗传进化分析 |
| |
| Isolation,Identification of Parvovirus from Canine in Lanzhou and Phylogeny Analysis of VP2 Gene Sequences |
|
|
| Abstract:To investigate the genotype distribution and genetic evolution of canine parvovirus (CPV) in Lanzhou in recent years, eight fecal samples tested positive using a canine parvovirus rapid test kit were collected from pet hospitals in Lanzhou. F81 cells were used for isolating parvovirus strains; PCR,indirect immunofluorescence and hemagglutination assays were conducted to identify the isolated strains; additionally,the genotype and genetic evolution relationship were analyzed. The results showed that seven parvovirus strains were isolated,one strain belonged to the New CPV-2a type,five strains were classified as CPV-2c type,and one strain was identified as Feline parvovirus (FPV). The homology of amino acid sequences and evolutionary analysis showed that the VP2 sequences of these CPV-2c strain was 100% homologous and closely related to the strains found in Beijing and Jiangsu. The New CPV-2a strain exhibited similarities to those found in Jilin and Xi’an. Compared with the reported strains,FPV of canine origin (LZ05) had unique mutations (91S→A and 322V→T),and was found to be most closely related to a strain isolated in Beijing in 2020. |
| keywords:Canine parvovirus Isolation and identification VP2 Genetic evolutionary analysis |
| 查看全文 查看/发表评论 下载PDF阅读器 |
