| Abstract:Boschniakia rossica is a valuable medicinal materials native to northeastern China,known for its therapeutic effect in strengthening kidney function,combating constipation, and promoting hemostasis.It contains active compounds such as Boschniakia rossica polysaccharides, Iridoid glucosides,Boschniakia rossica acid, all contributing to its significant medicinal value.To further explore the transcriptome of stanche from Daxing’an Mountains and enhance its genetic database, we conducted a full-length transcriptomic analysis of Boschniakia rossica seeds using high-throughput sequencing technology.Through transcriptomes squencing we obtained Unigene sequence,followed by functional annotation, SSR (Simple Sequence Repeat) analysis and CDS (Coding Sequence) prediction, and an in-depth analysis of genetic information.The results showed that the a total of 18.87 Gb of clean data was obtained, with the effective data per sample ranging from 5.4 to 7.06 Gb.The Q30 base quality score ranged from 95.66% to 97.45%, and the average GC content was 49.35%.We identified 57 799 Unigen sequnces, with a total length of 48 308 661 bp and an average length of 835.8 bp .The Boschniakia rossica seeds transcriptome showed the highest sequence similarity to Brassica napus.Functional annotations revealed that 47 035(81.38%) genes were annotated in the NR database, 33 653 (58.22%) in Swissprot, 13 174(22.79%) in KEGG, 27 886(48.25%) in KOG, 42 506(73.54%) in eggNOG, 30 928(53.51%) in GO, 29 642(51.28%) in Pfam.11 031 SSRs were predicted, with 57 799 Unigene containing SSRs, 1 886 Unigene containing more than 1SSRs, and compound SSRs 943 predicted.A total of 53 033 CDS sequences were predicted, with 47 140 were predicted by the database alignment and 5 893 by ESTScan.These findings enrich the transcriptome data of Boschniakia rossica, and provide a reference for further studying the biological characteristics and molecular mechanism of Boschniakia rossica.This study also lays a foundation for the sustainable exploitation, protection, and utilization of Boschniakia rossica resources. |
