| 红花玉兰嫩枝扦插生根的转录组和内源激素变化分析 |
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| 引用本文：周美琪,向旭初,桑子阳 ,陈发菊,梁宏伟.红花玉兰嫩枝扦插生根的转录组和内源激素变化分析[J].西北农业学报,2024,(11):2111~2123 |
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| 基金项目:2022年湖北省中央引导地方科技发展专项（2022BG E265）。 |
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| 中文摘要:为探究红花玉兰嫩枝扦插不定根发生的分子机制，利用1 000 mg/L的萘乙酸（NAA）处理当年生嫩枝进行扦插，分别对新采回枝条及插穗生根过程中不同阶段进行取样，开展RNA-Seq与内源激素水平分析。结果表明，（1）通过RNA-Seq总共组装了186 303个Unigene，其中88 161条Unigene得到注释，总注释率达到了47.32%。通过差异表达基因分析可知，相较于新采回插穗的阶段S0，在经NAA处理6 h后的S1、根原基突破表皮的S2和不定根伸长的S3阶段上调表达基因分别有5 796、3 205和4 262个，下调表达基因分别有3 795、5 248和6 305个；KEGG 富集分析显示这些基因大多聚集于苯丙素生物合成、植物激素信号转导、淀粉和蔗糖代谢等通路中，其差异表达可能与红花玉兰的扦插生根密切相关。（2）内源激素水平分析表明：与新采回插穗S0相比，S1阶段插穗中IAA、ABA、GA3和CTKs（IP和Zeatin）浓度均显著上升；在S2阶段IAA和ABA浓度显著降低，但IAA/ABA比值显著升高，GA3和IP浓度升至最高且与其他阶段相比差异显著，而Zeatin变化不明显；至不定根伸长的S3阶段IAA和ABA浓度显著回升，而GA3和CTKs浓度则显著下降。综合分析表明，生长素、脱落酸和细胞分裂素等内源激素的动态变化及其比值促进了根原基形成和不定根的伸长生长，植物激素信号转导相关基因的差异表达与内源激素水平的升降相一致，最终诱导红花玉兰嫩枝插条生根。 |
| 中文关键词:红花玉兰 扦插生根 转录组测序 内源激素 差异表达基因 |
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| Analysis of Transcriptome and Endogenous Hormone Changes in Rooting Process of Shoot Cuttings of Magnolia wufengensis |
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| Abstract:To explore the molecular mechanism underlying adventitious rooting in softwood cuttings of Magnolia wufengensis,1 000 mg/L naphthalene acetic acid (NAA) was used to treat softwood cuttings from the current year’s new growth.The cuttings were sampled were collected at different stages during rooting process of newly collected branches and cuttings.RNA-Seq and endogenous hormone levels were analyzed.The results showed that a total of 186 303 unigenes were assembled with RNA-Seq,and 88 161 of them were annotated,with an annotation rate of 47.32%.Based on the analysis of differentially expressed genes,significant differences in gene expression were observed between the newly harvested cuttings (referred to as stage S0) and the cuttings at stages S1,S2,and S3.In particular,5 796,3 205 and 4 262 genes were up-regulated and 3 795,5 248 and 6 305 genes were down-regulated in the cuttings at stage S1 (after 6 hours of treatment with NAA),stage S2 (i.e.,root primordium formation phase) and stage S3 (i.e.,adventitious root elongation phase),respectively.KEGG enrichment analysis showed that most of these genes were clustered in pathways such as phenylpropanoid biosynthesis,plant hormone signal transduction,starch and sucrose metabolism.The differential expression of these genes might be closely related to the rooting in the cuttings of Magnolia wufengensis.The analysis of endogenous hormone levels revealed that the concentrations of IAA,ABA,GA3 and CTKs (IP and Zeatin) in the cutting sample at stage S1 were significantly higher than those in the cutting sample at stage S0.At stage S2,the concentrations of IAA and ABA decreased significantly,but the ratio of IAA/ABA increased significantly.Furthermore,at stage S2,the concentrations of GA3 and IP increased and were significantly higher than those at other stages,but the change of Zeatin was not evident.At stage S3,the concentrations of IAA and ABA increased significantly,while the concentrations of GA3 and CTKs decreased significantly.The comprehensive analysis showed that the dynamic changes in endogenous hormones such as auxin,abscisic acid and cytokinin and in their ratios promote the formation of the root primordium and the elongation of adventitious roots.The differential expression of genes related to plant hormone signal transduction is consistent with the change of endogenous hormone levels,finally promoting the rooting of the softwood cuttings of Magnolia wufengensis. |
| keywords:Magnolia wufengensis Cuttage rooting RNA-Seq Endogenous hormone Differentially expressed genes |
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