| 驴源马链球菌马亚种的分离鉴定及生物学特性分析 |
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| 引用本文：李金荣，张 伟，郝宝山，王 馨，高函雅，徐李依娜，揭郑昊，赛福丁·阿不拉，阿得力江·吾斯曼，况 玲，刘丹丹.驴源马链球菌马亚种的分离鉴定及生物学特性分析[J].西北农业学报,2025,(2):337~345 |
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| 基金项目:新疆自治区青年基金（2023D01B31）；中央引导地方科技发展专项资金项目（ZYYD2023C03）；新疆农业大学研究生科研创新项目（XJAUGRI2023012)；2023年大学生创新项目（2022SJKF45）。 |
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| 中文摘要:为探究新疆喀什某驴场驴腺疫病的病原菌及其相关生物学特性，应用绵羊鲜血培养基对采集样品进行细菌的分离纯化，通过革兰氏染色、镜检及PCR扩增对分离菌进行鉴定，并利用 MEGA 7.0生物软件绘制分离菌的遗传进化树；绘制分离菌生长曲线，通过分离菌体内致病性试验、毒力基因检测试验以及药物敏感性试验等对分离菌进行生物学特性分析。结果显示，获得一株具有 β溶血特性且革兰氏染色阳性的链球菌，测序结果通过GenBank的Blast比对，与已注册马链球菌马亚种同源性高达99.01%，遗传进化树分析发现该分离菌与新疆马链球菌马亚种HTP133（MT815602.1）遗传距离最近，命名为LXY019株；从生长曲线可以看出该分离菌在培养8 h后达到平台期，18 h后走向衰亡；致病性试验结果显示，分离菌对小鼠的LD50为 1.58×107CFU/mL，毒力基因鉴定结果提示该分离菌携带fneB和seM；药敏试验结果显示该分离菌对 β-内酰胺类抗生素和大环内酯类抗生素高度敏感，对氨基苷类抗生素的敏感性相对较低。以上结果表明，分离纯化的驴源马链球菌马亚种对小鼠具有明显的致病性，临床治疗可优先选用 β-内酰胺类抗生素和大环内酯类抗生素，结果可以为驴源马链球菌马亚种感染引起的驴腺疫的防治提供参考。 |
| 中文关键词:驴腺疫 马链球菌马亚种 致病性 毒力基因 药物敏感性 |
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| Isolation and Identification of Strain of Streptococcus equi Subspecies. equi from Donkey and Analysis of Its Biological Characteristics |
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| Abstract:Toexplore the pathogenic bacteria and related biological characteristics of donkey strangles in a donkey farm in Kashgar,Xinjiang,bacteria from collected samples using sheep fresh blood culture medium were isolated and purified. The isolated bacteria were identified through Gram staining,microscopy,and PCR amplification. MEGA 7.0 biological software was used to draw the genetic evolution tree of isolated bacteria,and the growth curve of isolated bacteria was drawn and their biological characteristics were analyzed through conducting in vivo pathogenicity tests,virulence gene detection tests,and drug sensitivity tests. The results the isolation of a strain of streptococci with β-hemolytic properties and a positive Gram stain. The sequencing results were compared with GenBank’s Blast and showed a high homology of 99.01% with the registered Streptococcus equi subspecies. equi. The genetic evolution tree analysis indicated that the genetic distance between the isolates of Streptococcus equi subspecies. equi from the donkey and the isolates of Streptococcus equi subspecies. equi from Xinjiang HTP133(MT815602.1) was similar,the isolates were named LXY019 strain.The growth curve showed that the isolated bacteria reached a plateau period after 8 hours and began to decline after 18 hours. Pathogenicity results indicated that the LD50 of the isolated strain in mice was 1.58× 107 CFU/mL. Virulence gene identification indicated that the isolated strain carried fneB and seM. The final drug sensitivity test results showed that the isolated bacteria were sensitive to β-Lactam antibiotics and macrolide antibiotics were highly sensitive,while aminoglycoside antibiotics were relatively insensitive. These findings showed that the Streptococcus equi subspecies isolated and purified from donkeys exhibits obvious pathogenicity in mice,and β-lactam antibiotics and macrolide antibiotics are recommended for clinical treatment. The results provide a reference for the prevention and treatment of donkey strangles caused by this pathogen. |
| keywords:Donkey strangles Streptococcus equi subspecies. equi Pathogenicity Virulence genes Drug sensitivity |
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