| 小麦条锈菌（CYR34）全基因组剪接体PRP8家族基因的鉴定及表达分析 |
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| 引用本文：陈诗雯,韩 宏,杨成德,邹一萍,王增艳,王丽婷,陶 飞.小麦条锈菌（CYR34）全基因组剪接体PRP8家族基因的鉴定及表达分析[J].西北农业学报,2025,(4):617~627 |
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| 基金项目:国家自然基金地区科学基金（32060595）；甘肃省青年基金（20JR10RA549）；甘肃农业大学人才专项（GAU-KYQD-2018-37）。 |
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| 中文摘要:PRP8（precursor RNA processing 8）作为高度保守的剪接体蛋白之一，在剪接体的催化过程中占据中心位置，且在病原菌生长、发育及致病性中起重要的作用，研究小麦条锈菌主流小种CYR34中PRP8基因家族在应对全球气候变暖及致病性方面的作用，可为小麦条锈病的“绿色防控”成为潜在的药物靶点提供理论依据。利用TBtools软件和CYR34基因组注释文件（GFF文件）绘制染色体定位图、蛋白结构域、基因共线性和作用元件分析；通过Q-PCR分析PRP8基因家族受温度胁迫及侵染寄主过程中的组织学变化及时序表达模式。CYR34小种中PRP8基因家族编码的蛋白长度为366~2 384氨基酸，其中，PRP801编码的氨基酸有25个外显子，PRP802和PRP803外显子数目都为7个；4个PRP8都具有典型的PRP8 superfamily结构域，且位置相似；4个PRP8基因分别分布在CM045847.1、CM045850.1、CM045852.1和CM045862.1染色体上，且PRP802和PRP803为一对共线性基因；4个PRP8基因的启动子上均含有常见的顺式作用元件（CAAT-box、NON-box、CGTCA-motif、TATA-box和CAAT-box）；条锈菌CYR34小种中PRP802和PRP803和条锈菌Ps93XT-03和Ps93-210-02小种中的亲缘关系较近；在温度胁迫、侵染寄主过程中PRP801、PRP802和PRP803基因在6 h或12 h与对照有显著差异（P<0.05），且组织学观察发现菌丝长度和次生菌丝分枝数在侵染前12 h增速较快，分别为2 h时的2.62和3.31倍（P<0.05）。小麦条锈菌CYR34小种中PRP8基因家族存在4个PRP8基因，且以某种剪接模式在温度胁迫及侵染寄主过程发挥重要作用。 |
| 中文关键词:小麦条锈菌 PRP8 表达模式 生物和非生物胁迫 |
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| Identification and Expression Analysis of PRP8 Family Genes in Puccinia striiformis f.sp.tritici (CYR34) |
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| Abstract:Precursor RNA processing 8 (PRP8) isa highly conserved spliceosome component,essential for catalyzing pre-mRNA splicing and playing an important role in the pathogenicity,growth,and development of pathogens.This study investigated the role of the PRP8 gene family in CYR34,the predominant race of Puccinia striiformis f.sp.tritici (wheat stripe rust fungus),to elucidate its function in adapting to global climate change and enhancing pathogenicity.This study may provide theoretical support for developing “green control” methods for wheat stripe rust by identifying potential drug targets.Chromosome localization,protein domains,gene collinearity and cis-acting elements were analyzed using TBtools software and the CYR34 genome annotation file.Expression pattern of the PRP8 gene family under temperature stress and during host infection were examined through qPCR.In CYR34,four PRP8 proteins (366 to 2 384 amino acids in length) [JP3]were identified as PRP801 to PRP804.[JP]All four PRP8 proteins in CYR34 contained typical RPR8 superfamily domains in similar positions.The coding sequence of PRP801 contained 25 exons,while those of PRP802 and PRP803 contained 7 exons.The four PRP8 genes were distributed on chromosomes CM045847.1,CM045850.1,CM045852.1 and CM045862.1 respectively,among them,PRP802 and PRP803 were a pair of collinear genes.The promoters of all four PRP8 genes contained a large number of commonly found cis-acting elements (e.g.CAAT box,NON box,CGTCA motif,TATA box and CAAT box).Phylogenetic analysis showed that PRP802 and PRP803 of the CYR34 was relatively close to RPR9 in races Ps93XT-03 and Ps93-210-02 of Puccinia striiformis f.sp.tritici.During different temperature treatment and infection,the expression patterns of PRP801, PRP802 and PRP803 genes was significantly different from the control (P<0.05).Meanwhile,histopathological observation revealed that the length of hyphae and the number of secondary hyphal branches increased rapidly at 12 hours after infection,being 1.62 and 2.31 times higher than those at 2 hours post-infection respectively (P<0.05).The four PRP8 genes in the PRP8 family of CYR34 play an important role in response to temperature stress and during pathogenic invasion,possibly through a splicing pattern. |
| keywords:Puccinia striiformis f.sp.tritici PRP8 Gene expression Biological and abiotic stress |
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