| Abstract:To screen interspecific hybrids with intense fruit aroma between F.×ananassa and F.vesca, the cultivar ‘Sweet charlie’ was used as the female parent and crossed with wild accession Senlinbaiguo, 7 F1 progeny were obtained.The interspecific hybrid identification was conducted using a combination of morphological markers,flow cytometry, and SSR molecular markers.Fruit volatile compounds in the identified hybrids were qualitatively analyzed using Gas Chromatograph-Mass Spectrometry (GC-MS).The results showed distinct leaf characteristics in the F1 plants, with foliage that was green to dark green, and terminal leaflets that were round to oval, differentiating them from those of Senlinbaiguo.The offspring leaves were significantly thicker than those of F.vesca, and overall, leaf morphology in the F1 progeny were more closely resembled ‘Sweet charlie’, the F.×ananassa parent.Seven progeny exhibited sterility to varying degrees.All F1 fruits were pink or red upon ripening, with no white mature fruits observed.Five pentaploid F1 progeny and two hexaploid ones were identified by flow cytometry.The SSR primer Fv000003v1 amplified parent-specific bands in all seven lines.A PCR product about 400 bp was found in Senlinbaiguo and tested offspring plants usinggel electrophoresis, producing a PCR product of approximately 400 bp in Senlinbaiguo and the F1 plants, but not in ‘Sweet charlie’.This confirmed that the seven F1 progeny were interspecific hybrids.Fruit volatile compounds were detected in the parental lines and four hybrids.The result showed that the number of volatile compounds in the interspecific hybrids was higher than those in the parents.Specifically, the hybrids ‘21-509-2’ and ‘21-509-9’ showed increased concentrations of aroma-contributing volatiles, with compounds characteristic of F.vesca, such as ethyl decanoate, ethyl dodecanoate, 2-undecanone, and 2-tridecanone, enhancing their aromatic profiles.These two hybrids are suitable for further aroma breeding. |
