| 玫瑰压花花瓣酶促褐变机理分析及相关酶基因表达研究 |
| 点此下载全文 |
| 引用本文：周徐子鑫，黄郑涛，林 伟，刘 晴，马 均，陈国菊.玫瑰压花花瓣酶促褐变机理分析及相关酶基因表达研究[J].西北农业学报,2026,(3):471~482 |
| DOI: |
| 摘要点击次数: 217 |
| 全文下载次数: 54 |
|
| 基金项目:国家自然科学基金（31971704）；四川省自然科学基金（22NSFSC0090）；宜宾职业技术学院自然科学项目（24ZRYB-14）。 |
|
| 中文摘要:为了减轻红色玫瑰品种‘卡罗拉’(Rosa rugosa ‘Carola’)花瓣在压花过程中的褐变程度，利用理化分析和转录组学挖掘出在玫瑰压花过程中影响酶促褐变的主要多酚氧化酶(PPO)基因，并依据其启动子元件信息探究了脱落酸(ABA)和茉莉酸甲酯(MeJA)对玫瑰压花花瓣酶促褐变的作用。结果表明：(1)‘卡罗拉’花瓣在压花过程中总酚和总黄酮含量降低，多酚氧化酶（PPO）、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性升高，表明花瓣在压花过程中存在显著的酶促褐变；(2)比较转录组与qRT-PCR结果表明 RrPPO1、 RrPPO3和 RrPPO5的表达水平在压花过程中显著上调，皮尔森相关性系数表明三者的表达水平与多酚氧化酶活性变化存在显著正相关，启动子分析表明三者启动子上主要的化学激素响应元件为ABA响应元件和MeJA响应元件；(3)与对照相比，经ABA或MeJA处理后的玫瑰压花花瓣中PPO活性均显著降低，MeJA对PPO活性抑制作用比ABA更加显著，而ABA在抑制PPO活性的同时能够有效激活超氧化物歧化酶和过氧化氢酶（CAT）的活性及其编码成员的表达。 |
| 中文关键词:压花艺术 玫瑰 酶促褐变 多酚氧化酶 转录组 |
| |
| Analysis of Mechanism of Enzymatic Browning in Pressed Rose Petals and Expression of Related Enzyme Genes |
|
|
| Abstract:To alleviate enzymatic browning in petals of the red rose cultivar ‘Carola’ (Rosa rugosa ‘Carola’) during the flower the pressing process,this study employed physicochemical analysis and transcriptomic profiling to identify the major polyphenol oxidase (PPO) genes involved.Based on the analysis of promoter cis-elements,the study also investigated the effects of abscisic acid (ABA) and methyl jasmonate (MeJA) on enzymatic browning in pressed rose petals.The results showed that: (1) During the process pressing,the total phenol and total flavonoid contents decreased,while the activities of polyphenol oxidase (PPO),peroxidase (POD),and superoxide dismutase (SOD) significantly increased,indicating pronounced enzymatic browning; (2) Comparative analysis of transcriptomic and qRT-PCR data showed that the expression levels of RrPPO1, RrPPO3,and RrPPO5 were significantly upregulated during the flower pressing process.Pearson correlation analysis indicated a significant positive correlation between the expression levels of these genes and PPO activity.Promoter analysis revealed that the major hormone-responsive elements in the promoters of these genes were ABA- and MeJA-responsive elements; (3) Compared with the control,PPO activity was significantly reduced in petals treated with either ABA or MeJA,with MeJA exhibiting a stronger inhibitory effect.Additionally,ABA treatment not only suppressed PPO activity but also enhanced the activities and expression of SOD and catalase (CAT). |
| keywords:Pressed flower art Rosa rugosa Enzymatic browning Polyphenol oxidase Transcriptome |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
|
