| 马铃薯蛋白激酶基因 StSnRK1通过抑制PTI免疫反应负调控植物对致病疫霉的抗性研究 |
| 点此下载全文 |
| 引用本文：刘泽文，丁俐文，汪炜杭，萨 仁，秦亚岚，王红梅，孟玉玲，单卫星.马铃薯蛋白激酶基因 StSnRK1通过抑制PTI免疫反应负调控植物对致病疫霉的抗性研究[J].西北农业学报,2026,(3):540~547 |
| DOI: |
| 摘要点击次数: 220 |
| 全文下载次数: 62 |
|
| 基金项目:国家自然科学基金（31930094）。 |
|
| 中文摘要:本研究聚焦马铃薯蔗糖非发酵-1-相关蛋白激酶1（sucrose non-fermenting-1-related protein kinase 1,SnRK1）（StSnRK1），通过RT-qPCR分析其响应致病疫霉侵染的表达模式，利用根癌农杆菌（Agrobacterium tumefaciens）介导的瞬时表达技术，结合致病疫霉侵染测试，初步分析其对本氏烟抗病性的影响及机制。结果显示， StSnRK1在致病疫霉侵染后期上调表达，过表达 StSnRK1抑制本氏烟对致病疫霉的抗性。进一步分析发现，过表达 StSnRK1抑制致病疫霉模式分子INF1引发的细胞死亡、细菌模式分子flg22诱导的活性氧迸发和胼胝质沉积等PTI反应。综上所述，本研究初步揭示 StSnRK1通过抑制PTI免疫反应，负调控植物对致病疫霉的抗性。 |
| 中文关键词:马铃薯 致病疫霉 StSnRK1 植物免疫 |
| |
| StSnRK1 Negatively Regulates Plant Resistance to Phytophthora infestans by Inhibiting PTI Responses |
|
|
| Abstract:In this study,we investigated the role of potato SnRK1 ( StSnRK1)in plant immunity and its expression dynamics during P. infestans infection. Reverse transcription quantitative PCR (RT-qPCR) was used to examine its expression pattern,and Agrobacterium tumefaciens-mediated transient gene expression in Nicotiana benthamiana was conducted to assess its function,followed by inoculation with P. infestans. The results showed that StSnRK1 was upregulated in the late stage of P. infestans infection. Overexpression of StSnRK1 reduced N. benthamiana resistance to P. infestans. In addition,overexpression of StSnRK1 inhibited pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) responses,including INF1-induced cell death,flg22-triggered reactive oxygen species (ROS) burst,and callose deposition. In summary,this study demonstrates that the potato protein kinase gene StSnRK1 negatively regulates plant resistance to P.infestans by suppressing PTI responses. |
| keywords:Potato Phytophthora infestans StSnRK1 Plant immunity |
| 查看全文 查看/发表评论 下载PDF阅读器 |
